芒果苷对同型半胱氨酸诱导骨髓间充质干细胞分化的影响

doi:10.3969/j.issn.1006-5725.2024.23.002

Abstract

Abstract:

Objective To establish a lipid differentiation model of primary rat bone marrow mesenchymal stem cells （rBMSCs） in vitro using homocysteine （Hcy）， and analyze the specific effects of Hcy on lipid and bone differentiation of BMSCs； to comprehensively explore the effects of mangiferin on lipid and bone differentiation of BMSCs， and further reveal the potential mechanism of mangiferin in the treatment of osteoporosis through the intervention of Hcy-induced BMSCs by different concentrations of mangiferin. Methods First， rBMSCs were extracted and isolated. The rBMSCs that were well-cultured to a certain generation were placed in culture medium containing different concentrations of Hcy （0.125， 0.250， 0.5， 1， 2， 4 mmol/L） to establish lipid differentiation model of rBMSCs. Then， different concentrations of mangiferin （37.5， 75， 150 μmol/L） were applied to the experimental cells for intervention. After culture for a certain period of time， the cells were collected for the following tests： The accumulation of lipids in the cells was detected by semi-quantitative method of oil red O dye solution to evaluate the degree of lipid differentiation； The activity of alkaline phosphatase （ALP） was measured by ρNPP method； The expression of bone morphogenetic protein-2 （BMP-2） and type I collagen （ColⅠ） were detected by western blot assay to evaluate the degree of bone differentiation. Results Mangiferin could significantly up-regulate the expression of BMP-2 and ColⅠ in vitro， increase the level of ALP， and promote bone differentiation of rBMSCs. Hcy promoted lipid differentiation of rBMSCs by increasing lipid accumulation， and down-regulated the expression of BMP-2 and ColⅠ， reduced the intracellular ALP level， thereby inhibiting bone differentiation of rBMSCs. However， the above Hcy-related effects could be successfully reversed by mangiferin. Conclusion Mangiferin can significantly promote bone differentiation of rBMSCs in vitro， while Hcy can inhibit bone differentiation of rBMSCs and promote its lipid differentiation. Mangiferin has the ability to reverse this effect， indicating that mangiferin has certain potential in the treatment of osteoporosis-related diseases.

Key words: BMSC, mangiferin, homocysteine, lipid-differentiation, bone-differentiation

CLC Number:

R285

Suzhen CHAO,Nian ZHOU,Xinyi SHI,Yili ZHOU,Junjie XIA,Bo LIU,Mingshi REN,Zihan. LI. Effect of mangiferin on differentiation of bone marrow mesenchymal stem cells induced by homocysteine[J]. The Journal of Practical Medicine, 2024, 40(23): 3284-3290.

Figures/Tables 8

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Effect of mangiferin on differentiation of bone marrow mesenchymal stem cells induced by homocysteine

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