目的 观察香叶木素对 PC⁃3 细胞增殖、凋亡、集落形成、迁移、黏附及干性的影响。方法 利用 CCK8 检测香叶木素对前列腺癌细胞 PC⁃3 的增殖情况，并计算其半抑制值（IC50）；在显微镜下观察香 叶木素对 PC⁃3 细胞形态的影响，并用 Western blot 检测凋亡相关基因的表达；分别采用细胞克隆形成实 验、Transwell 实验和黏附实验评估 PC⁃3 细胞集落形成、迁移及黏附能力的影响；最后利用肿瘤成球培养检 测 PC⁃3 细胞的更新能力。结果 香叶木素呈剂量效应抑制 PC⁃3 的生长，差异有统计学意义（P < 0.05）， 其 IC50约为 10 μg/mL。香叶木素能诱导 PC⁃3 细胞发生凋亡，促进凋亡相关基因 Caspase⁃8、Bax 表达同时抑 制抗凋亡基因 Bcl⁃2 表达；此外，香叶木素能在体外抑制 PC⁃3 细胞的集落形成、迁移、黏附及成球能力 （P < 0.05）。结论 香叶木素在体外能抑制 PC⁃3 细胞的增殖、集落形成、迁移、黏附能力及干性，同时能诱 导PC⁃3细胞发生凋亡。

Objective To evaluate the effect of Diosmetin on proliferation，apoptosis，colony formation， migration，adhesion and stemness of PC⁃3cells. Methods The effect of Diosmetin on PC⁃3 cells proliferation was detected using CCK⁃8 assay，and the half maximal inhibitory concentration（IC50）value of Diosmetin was calculated. Cell morphology was observed under a microscope，and the apoptotic⁃related proteins were detected using WB as aforementioned. The ability of colony ⁃forming，adherence and migration of PC ⁃3 cells were determined by colony formation assay，cell adhesion experiment and Transwell experiment，respectively. Results Diosmetin inhibited the growth of PC⁃3 cells in a dose⁃dependent manner，with statistically significant difference（P < 0.05），and the results showed an IC50 value of 10 μg/mL. At the same time，Diosmetin induced PC⁃3 cell apoptosis，western blot analysis demonstrated that the expression of the apoptosis⁃associated genes caspase⁃8，Bax was upregulated while Bcl⁃2 was downregulated. In addition，Diosmetin suppressed PC⁃3 cells colony formation，adhesion，migration and sphere ⁃formingabilities，with statistically significant the difference（P < 0.05）. Conclusion Diosmetin inhibited proliferation，colony formation，adhesion，migration abilities and stemness of PC⁃3 cellsin vitro. Meanwhile，Dios⁃ metin induces apoptosis of PC⁃3 cells.