目的 研究miR⁃29a 靶向信号转导及转录激活因子3（signal transduction and activator oftranscription 3，STAT3）调控炎症反应在脂多糖（LPS）诱导大鼠肾小管上皮细胞损伤中的保护作用。方法 培养大鼠肾小管上皮细胞株NRK⁃52E，LPS刺激建立脓毒症细胞模型、诱导细胞损伤，转染miR⁃NC、miR⁃29a、pcDNA3.1质粒、pcDNA3.1⁃STAT3质粒或用STAT3抑制剂AG490处理，比较细胞活力A490、STAT3、p⁃STAT3、bcl⁃2表达量，TNF⁃α、IL⁃1β含量的差异；双荧光素酶报告基因验证miR⁃29a靶向STAT3。结果 LPS组的miR⁃29a表达水平、A490水平、Bcl⁃2的表达水平均低于对照组，STAT3、p⁃STAT3的表达水平、TNF⁃α、IL⁃1β的含量均高于对照组；LPS+miR⁃29a 组的miR⁃29a 表达水平、A490 水平、Bcl⁃2 的表达水平均高于LPS 组，STAT3、p⁃STAT3的表达水平、TNF⁃α、IL⁃1β的含量均低于LPS组；pcDNA3.1⁃STAT3+miR⁃29a组的A490水平及Bcl⁃2的表达水平均低于pcDNA3.1+miR⁃29a组，STAT3、p⁃STAT3的表达水平、TNF⁃α、IL⁃1β的含量均高于pcDNA3.1+miR⁃29a组；miR⁃29a组STAT3双荧光素酶报告基因的荧光活力低于miR⁃NC组。结论 STAT3通路激活与LPS诱导的肾小管上皮细胞炎症反应及凋亡有关，miR⁃29a通过靶向STAT3减轻LPS引起的炎症反应及凋亡。

Objective To study the protective effect of miR⁃29a on LPS induced rat renal tubular epithelialcell injury by targeting signal transduction and activator of transcription 3（STAT3）. Methods Rat renal tubularepithelial cell line NRK⁃52E was cultured. LPS stimulation was used to establish sepsis cell model and induce cellinjury，then transfected with miR⁃nc，miR⁃29a，pcDNA3.1 plasmid，pcDNA3.1⁃STAT3 plasmid or treated withSTAT3 inhibitor AG490. Then cell viability A490，the expression of STAT3，p⁃STAT3 and Bcl⁃2，TNF⁃α andIL⁃β contents were compared，and miR⁃29a targeting STAT3 was confirmed by double luciferase reporter gene.Result The expression levels of miR⁃29a，Bcl⁃2，the level of A490 of LPS group were lower than those of the con⁃trol group，while the expression of STAT3，p⁃STAT3，the contents of TNF⁃α and IL⁃β were higher than those incontrol group；the expression levels of miR⁃29a，Bcl⁃2，the level of A490 of LPS+miR⁃29a group were higher thanthose of the LPS group，while the expression of STAT3，p⁃STAT3，the contents of TNF⁃α and IL⁃β were lowerthan those in LPS group；the expression levels of Bcl⁃2，the level of A490 of pcDNA3.1⁃STAT3+miR⁃29a were low⁃er than pcDNA3.1+miR⁃29a group，while the expression of STAT3，p⁃STAT3，the contents of TNF⁃α and IL⁃βwere higher than those in LPS group；the fluorescence activity of STAT3 double luciferase reporter gene of miR⁃29agroup was lower than that of miR⁃nc group. Conclusion STAT3 pathway activation is related to LPS inducedinflammatory response and apoptosis of renal tubular epithelial cells，miR⁃29a can reduce LPS induced inflammatoryresponse and apoptosis by targeting STAT3.