金丝桃苷对胶原诱导性关节炎小鼠的治疗作用

金向楠 高薇 冯欣 隋海娟 付强

doi:10.3969/j.issn.1006⁃5725.2021.17.006

实用医学杂志 >

2021 , Vol. 37 >Issue 17: 2199 - 2203

DOI: https://doi.org/10.3969/j.issn.1006⁃5725.2021.17.006

基础研究

金丝桃苷对胶原诱导性关节炎小鼠的治疗作用

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锦州医科大学附属第一医院1风湿免疫科，3骨科（辽宁锦州121000）；2锦州医科大学基础医学药理学教研室（辽宁锦州121000）

网络出版日期: 2021-09-10

基金资助

辽宁省自然科学基金项目（编号：2019⁃ZD⁃0608）

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Therapeutic effect of Hyperoside on mice with collagen⁃induced arthritis

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Department of Rheumatoid Immunity，the First Affiiated Hospital of Jinzhou Medi⁃ cal University，Jinzhou 121000，China

Online published: 2021-09-10

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摘要

目的观察金丝桃苷（hyperoside，Hyp）对胶原诱导性关节炎小鼠（CIA）的治疗作用。方法将DBA/1小鼠随机分为正常对照组10只，模型组35只，模型组在第0天及第21天分别给予牛Ⅱ型胶原与弗氏完全佐剂的乳化剂，制备CIA模型。第31天正常对照组随机选取8只，模型组进一步随机分组为模型对照组，金丝桃苷25mg/kg和50mg/kg治疗组分别8只，并开始给药物处理。动态观察小鼠的足掌厚度测量及临床关节炎指数变化。第52天处死小鼠，HE染色观察踝关节组织病理变化，ELISA测定小鼠血清肿瘤坏死因子α（TNF⁃α）和白细胞介素6（IL⁃6）的含量。结果成功建立CIA模型，在第28天开始测量足掌厚度并进行临床关节炎指数评分，金丝桃苷治疗组足掌厚度及临床关节炎指数评分均降低。ELISA测细胞因子，治疗组测得血清中TNF⁃α及IL⁃6的含量较模型组明显降低，差异有统计学意义。组织病理观察结果显示，与模型组相比，治疗组小鼠踝关节滑膜增生、炎症细胞浸润情况均明显减轻。结论金丝桃苷对类风湿关节炎具有治疗作用

关键词：

金丝桃苷

; 胶原诱导性关节炎; 类风湿关节炎; 抗炎; 促炎细胞因子

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金向楠高薇冯欣隋海娟付强 .

金丝桃苷对胶原诱导性关节炎小鼠的治疗作用

[J]. 实用医学杂志, 2021 , 37(17) : 2199 -2203 . DOI: 10.3969/j.issn.1006⁃5725.2021.17.006

Abstract

Objective To observe the Hyperoside（Hyp）therapeutic effect of collagen ⁃induced arthritis （CIA）in mice. Methods The DBA/1 mice were randomly divided into normal control group（n = 10 mice per group）and model group（n = 35 mice per group）. To induce the CIA model，the model group were given bovine type Ⅱ collagen and complete Freund adjuvant emulsifier on day 0 and day 21，the normal control group was injected with saline. To examine the therapeutic effect of hyperoside，on day 31 arthritic mice were randomly divided into three groups（n = 8 mice per group）including the CIA group and hyperoside⁃treated group（25 mg/kg and 50 mg/kg）. The measurement of paw thickness and the changes of clinical arthritis index in mice were dynamically observed. On the day 52，the mice were killed，the pathological changes of ankle joint were observed by HE staining. The serum levels of tumor necrosis factor⁃α（TNF⁃α）and interleukin⁃6（IL⁃6）were measured by ELISA. Results CIA model was successfully established，then to measure the paw thickness and clinical arthritis index scores on the day 28. The paw thickness and clinical arthritis index scores were significantly decreased in hypero⁃ side⁃treated group（P < 0.01）. Cytokines were measured by ELISA，the contents of serum TNF⁃α and IL⁃6 in the hyperoside ⁃treated group were significantly lower than those in the CIA group，with significant statistical signifi⁃ cance. Histopathological observation results showed that compared with the CIA group，the synovial hyperplasia and inflammatory cell infiltration of the ankle joint in the hyperoside ⁃treated group were significantly reduced. Conclusion Hyperoside may have a therapeutic effect on rheumatoid arthritis.

Key words：

Hyperoside

; collagen?induced arthritis; rheumatoid arthritis; anti?inflammatory; pro?inflmmatory cytokine

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