目的 探讨常见过敏原致过敏反应易感性与过敏性皮肤病患者外周血 HLA DRB1*0901 （rs2395185）基因多态性的关系。方法 选择广州地区皮肤科就诊患者 304 例，采用免疫印迹法检测患者血清 19 种过敏原，PCR 测序检测外周血 HLA DRB1*0901 位点基因型，分析 HLA DRB1*0901 基因多态性与 过敏原致过敏反应发病风险的关系。结果 户尘螨过敏原检测结果阳性、阴性患者间 rs2395185 的 G/G 基 因型、G/T 基因型分布差异有统计学意义（P < 0.05），G/G、G/T 基因型与户尘螨过敏的发病风险有关（OR＝ 2.940，95%CI：1.167～7.407；OR＝2.889，95%CI：1.136～7.348）。轻度过敏与中重度过敏患者的 G/T 基因型分布差异有统计学意义（P < 0.05），G/T 基因型是过敏原反应严重程度的危险因素（OR＝8.681，95% CI：1.077～69.989）。结论 广州地区居民户尘螨皮肤过敏发病风险可能与其外周血 HLA DRB1*09：01 （rs2395185）位点 G/G、G/T 多态性有关，19 种过敏原过敏的严重程度可能与 rs2395185 位点 G/T 多态性 有关

Objective To investigate the relationship between susceptibility to allergic reaction induced by common allergens and HLA DRB1*0901（RS2395185）gene polymorphism in peripheral blood of patients with allergic skin disease. Methods A total of 304 patients who accepted treatment in department of dermatology in Guangzhou were selected. Nineteen kinds of allergens were detected by Western blotting. HLA DRB1 * 0901 geno⁃ types were detected by PCR sequencing. The relationship between HLA DRB1 * 0901 gene polymorphism and the risk of allergic reaction induced by allergens was analyzed. Results The G/G and G/T genotypes of rs2395185 were significantly different between the positive and negative patients（P < 0.05），and might be associated with the risk of house dust mite allergy（OR＝2.940，95%CI：1.167～7.407；OR＝2.889，95%CI：1.136～7.348）. There was significant difference in the distribution of G/T genotype between mild and moderate to severe allergic patients （P < 0.05）. G/T genotype may be a risk factor for the severity of allergic reaction（OR＝8.681，95%CI：1.077~ 69.989）. Conclusion The risk of skin allergy caused by Dermatophagoides pteronyssinus in population of Guang⁃ zhou may be related to the G/G and G/T polymorphisms of HLA DRB1 * 09：01（rs2395185），and the severity of allergy to 19 allergens may be related to the G/T polymorphism of rs2395185.