目的 探讨电针心包经“内关”“间使”穴对APP/PS1小鼠脑功能网络连接的影响。 方法 选用5月龄SPF级雄性小鼠共18只，其中携带APP/PS1双转基因的模型小鼠12只，按随机数字表法均分为两组：模型组（n = 6）和电针组（n = 6），另取遗传背景相同的C57BL/6小鼠6只设为对照组。电针组小鼠于“内关”“间使”穴给予电针干预，电针仪接同侧穴位，两侧穴位交替使用，隔日1次，共5周。电针结束后采用Morris水迷宫检测各组小鼠空间学习记忆能力，采用新物体识别观察各组小鼠的非空间依赖的记忆能力；以双侧海马为感兴趣区（ROI），采用静息态功能磁共振成像（rs-fMRI）分析海马与全脑功能连接情况；采用ELISA检测海马与前额叶皮层去甲肾上腺素（NE）、多巴胺（DA）含量变化；采用Western blot检测海马糖原合成酶激酶3β（GSK-3β）、突触蛋白I（SynI）表达水平。 结果 与对照组相比，模型组小鼠潜伏期延长（P < 0.01），穿越平台次数及目标象限探索时间均下降（P < 0.01），认知指数降低（P < 0.01）；与模型组相比，电针组小鼠潜伏期缩短（P < 0.01），穿越平台次数及目标象限探索时间均增加（P < 0.01），认知指数升高（P < 0.01）。与对照组相比，模型组小鼠右侧海马与左侧后梨状区过渡区域、右侧中央杏仁核、左侧齿状回、右侧内嗅区、右侧主嗅球功能连接降低，左侧海马与右侧后梨状区过渡区域、左侧主嗅球、左侧内嗅区功能连接降低；与模型组相比，电针组小鼠右侧海马与左侧后梨状区过渡区域、右侧中央杏仁核、右侧后梨状区过渡区域、左侧齿状回、右侧主嗅球、左侧主嗅球功能连接升高，左侧海马与右侧外侧隔核、右侧海马亚CA3、左侧主嗅球、左侧内嗅区功能连接升高。与对照组相比，模型组小鼠海马NE、DA含量降低（P < 0.05），前额叶皮层NE、DA含量降低（P < 0.05）；与模型组相比，电针组小鼠海马NE、DA含量升高（P < 0.05），前额叶皮层NE、DA含量升高（P < 0.05）。与对照组相比，模型组小鼠海马GSK-3β表达增多、SynI表达减少（P < 0.05），与模型组相比，电针组小鼠海马GSK-3β表达减少、SynI表达增多（P < 0.05）。 结论 电针心包经穴可恢复APP/PS1小鼠海马与其他脑区的功能连接情况，其机制可能与调控AD关键脑区的神经递质紊乱有关。

Objective To explore the effect of electroacupuncture at the Neiguan and Jianshi acupoints on the brain functional network connectivity in APP/PS1 mice. Methods A total of 18 5-month-old SPF-grade male mice were selected， among which 12 model mice carrying the APP/PS1 double transgenic were randomly divided into two groups according to the random number table method： the model group （n = 6） and the electroacupuncture group （n = 6）. Additionally， 6 C57BL/6 mice with the same genetic background were selected as the control group. The mice in the electroacupuncture group were given electroacupuncture intervention at the "Neiguan" and "Jianshi" acupoints. The electroacupuncture device was connected to the acupoints on the same side， and the acupoints on both sides were alternately used once every other day for a total of 5 weeks. After electroacupuncture， the spatial learning and memory ability of mice in each group was detected by Morris water maze， and the non-spatially dependent memory ability of mice in each group was observed by new object recognition. Taking the bilateral hippocampus as the region of interest （ROI）， resting-state functional magnetic resonance imaging （rs-fMRI） was used to analyze the functional connectivity between the hippocampus and the whole brain. The changes in the contents of norepinephrine （NE） and dopamine （DA） in the hippocampus and prefrontal cortex were detected by ELISA. The expression levels of glycogen synthase kinase 3β （GSK-3β） and synaptic protein I（SynI） in the hippocampus were detected by Western blot. Results Compared with the control group， the latency of mice in the model group was prolonged （P < 0.01）， the number of platform crossings and the exploration time of the target quadrant decreased （P < 0.01）， and the cognitive index decreased （P < 0.01）. Compared with the model group， the latency of mice in the electroacupuncture group was shortened （P < 0.01）， the number of platform crossings and the exploration time of the target quadrant were both increased （P < 0.01）， and the cognitive index was elevated （P < 0.01）. Compared with the control group， the functional connections of the transition area between the right hippocampus and the left posterior piriform region， the right central amygdala， the left dentate gyrus， the right entorhinal region， and the right main olfactory bulb in the model group mice were decreased. Compared with the model group， the functional connections of the transition area between the right hippocampus and the left posterior piriform region， the right central amygdala， the transition area of the right posterior piriform region， the left dentate gyrus， the right main olfactory bulb， and the left main olfactory bulb in the electroacupuncture group were increased. The functional connections between the left hippocampus and the right lateral nucleus， the right hippocampal subCA3， the left main olfactory bulb， and the left entorhinal area were elevated. Compared with the control group， the contents of NE and DA in the hippocampus of mice in the model group decreased （P < 0.05）， and the contents of NE and DA in the prefrontal cortex decreased （P < 0.05）. Compared with the model group， the contents of NE and DA in the hippocampus of mice in the electroacupuncture group increased （P < 0.05）， and those in the prefrontal cortex increased （P < 0.05）. Compared with the control group， the expression of GSK-3β in the hippocampus of mice in the model group increased and the expression of SynI decreased （P < 0.05）. Compared with the model group， the expression of GSK-3β in the hippocampus of mice in the electroacupuncture group decreased and the expression of SynI increased （P < 0.05）. Conclusions Electroacupuncture at the pericardium acupoint can restore the functional connectivity between the hippocampus and other brain regions in APP/PS1 mice. The mechanism may be related to the disorder of neurotransmitters regulating the key brain regions of AD.