目的 探讨吲哚菁绿荧光染色法应用于胸腔镜解剖性肺段切除术的可行性和安全性。 方法 选择2024年1月至2025年5月在我院行胸腔镜肺段切除术的患者，根据患者术中识别段间平面是否采用荧光染色法分为观察组（n = 112）和对照组（n = 98），对照组患者采用膨胀萎陷法。两组在性别、年龄、吸烟史、COPD病史、靶段、肺结节大小、肺结节CTR值、肺结节性质方面差异均无统计学意义（P > 0.05）。回顾性分析两组患者手术及并发症情况。 结果 手术情况：观察组和对照组术中出血量［（74.2 ± 13.8）mL vs. （74.9 ± 15.4）mL，P = 0.674］、是否存在胸腔黏连［（11.6% vs. 17.3%），P = 0.236］、淋巴结清扫组数［（2.0 ± 0.8）组 vs. （2.0 ± 0.8）组，P = 0.965］、结节与切缘距离［（3.0 ± 1.5）cm vs. （3.0 ± 1.4）cm，P = 0.667］、术后住院时间［（3.5 ± 1.2）d vs. （3.5 ± 1.2）d，P = 0.721］差异无统计学意义。而手术时间［（87.4 ± 14.1）min vs. （100.7 ± 14.3）min，P < 0.001］和段间平面出现时间［（11.0 ± 2.0）s vs. （962.7 ± 101.3）s，P < 0.001］差异有统计学意义，观察组段间平面持续时间为（103.4 ± 37.4）s，两组肺结节R0切除率均为100%。并发症情况：观察组和对照组在咯血（8.9% vs. 8.2%，P = 0.843）、肺不张（4.5% vs. 6.1%，P = 0.591）、肺部感染（3.6% vs. 9.2%，P = 0.092）、心律失常（2.7% vs. 7.1%，P = 0.130）、持续性肺漏气（10.7% vs. 16.3%，P = 0.233）等并发症的发生率方面差异均无统计学意义。 结论 相比传统的膨胀萎陷法，吲哚菁绿荧光法在术中可以快速、准确、清晰地显示段间平面，缩短手术时间，为胸腔镜解剖性肺段切除术提供可靠的技术保障，是一种安全可行的方法，值得在临床推广应用。

Objective To investigate the feasibility and safety of indocyanine green fluorescent staining method for thoracoscopic anatomical lung segmentectomy. Methods Patients who underwent thoracoscopic anatomical lung segmentectomy in our hospital from January 2024 to May 2025 were divided into experimental group （n = 112） and control group （n = 98）. Patients in the experimental group were adopted the indocyanine green fluorescent staining method in defining the intersegmental plane. Patients in the control group were adopted inflation-deflation method. There were no statistical differences between the two groups in sex， age， smoking history， COPD history， target lung segment， size of pulmonary nodules， CTR value of pulmonary nodules and nature of pulmonary nodules （P > 0.05）. Surgery and complications in both groups were retrospectively analyzed. Results Surgery： Intraoperative blood loss ［（74.2 ± 13.8）mL vs. （74.9 ± 15.4）mL， P = 0.674］， pleural adhesion ［（11.6% vs. 17.3%， P = 0.236］， the number of lymph node dissection ［（2.0 ± 0.8） groups vs. （2.0 ± 0.8） groups， P = 0.965］， distance from nodule and margin ［（3.0 ± 1.5）cm vs. （3.0 ± 1.4）cm， P = 0.667］， the time in hospital after operation ［（3.5 ± 1.2）d vs. （3.5 ± 1.2）d， P = 0.721］ in the experimental group and control group without any statistically difference. However， there were statistically significant differences between two groups in the operation time ［（87.4 ± 14.1）min vs. （100.7 ± 14.3）min， P < 0.001］ and the time of intersegment plane appearance ［（11.0 ± 2.0）s vs. （962.7 ± 101.3）s， P < 0.001］. and the duration of the intersegment plane in the experimental group was （103.4 ± 37.4）s. The R0 resection rate of pulmonary nodules was 100% in both groups. Complications： Two groups had no statistical differences in the incidence of hemoptysis （8.9% vs. 8.2%， P = 0.843）， atelectasis （4.5% vs. 6.1%， P = 0.591）， pulmonary infection （3.6% vs. 9.2%， P = 0.092）， arrhythmia （2.7% vs. 7.1%， P = 0.130）， and persistent pulmonary air leakage （10.7% vs. 16.3%， P = 0.233）. Conclusion Compared with the traditional inflation-deflation method， indocyanine green fluorescent staining method can quickly， accurately and clearly display the intersegment plane， shorten the operation time， and provide reliable technical support for thoracoscopic anatomical lung segment resection. It is a safe and feasible method， worthy of application in clinical practice.