收稿日期: 2025-11-20
网络出版日期: 2026-03-09
基金资助
广东省医学科学技术研究基金项目(B2023044);广东省中医药局科研项目(20232001)
TFAM overexpression promotes the proliferation and invasion of renal cancer cells
Received date: 2025-11-20
Online published: 2026-03-09
目的 探讨线粒体转录因子A（TFAM）基因对肾癌细胞增殖和侵袭的影响。 方法 在肾癌细胞ACHN、A704中高表达TFAM，通过流式细胞仪检测细胞凋亡。在ACHN、A704中低表达TFAM，通过CCK8、流式细胞仪、侵袭、迁移实验检测细胞增殖、周期和侵袭能力。通过qPCR、Western blot检测高表达TFAM和敲低TFAM后mRNA和蛋白的变化以及CHIP-qPCR探究TFAM靶基因。 结果 在ACHN、A704细胞中转染入质粒后，与对照组相比TFAM表达明显增高，高表达TFAM组细胞凋亡明显减少。在ACHN、A704细胞中敲低TFAM，与对照组相比TFAM明显降低，低表达TFAM细胞增殖能力、侵袭能力较对照组明显降低。高表达TFAM肾癌细胞增殖能力增强，低表达TFAM细胞增殖侵袭能力受到抑制。TFAM能靶向调控CDC25B和CCNE2，可能通过激活CDC25B和CCNE2促进肾癌细胞的增殖。 结论 TFAM能够促进肾癌细胞的增殖和侵袭。
李环辉 , 邱晓拂 , 李高远 , 付钰 . 高表达线粒体转录因子A对肾癌细胞增殖和侵袭的影响[J]. 实用医学杂志, 2026 , 42(5) : 759 -767 . DOI: 10.3969/j.issn.1006-5725.2026.05.005
Objective To conduct a focused investigation on the effect of the TFAM gene on the proliferation and invasion of renal cancer cells. Methods Renal cancer cells ACHN and A704 were transfected with a plasmid for TFAM overexpression， and cell apoptosis was detected by flow cytometry. In ACHN and A704 cells， TFAM was knocked down， and cell proliferation， cell cycle， and invasion ability were assessed using CCK8， flow cytometry， invasion， and migration assays. Changes in mRNA and protein levels after TFAM overexpression and knockdown were detected by qPCR and western blot， respectively. qPCR and western blot were used to detect mRNA and protein changes after TFAM overexpression and knockdown， and ChIP-qPCR was performed to explore TFAM target genes. Results After transfection with the plasmid， ACHN and A704 cells exhibited a significant increase in TFAM expression compared to the control group， and the apoptosis of cells in the high TFAM expression group was significantly reduced. When TFAM was knocked down in ACHN and A704 cells， TFAM levels were significantly lower than those in the control group， and the proliferation and invasion abilities of cells with low TFAM expression were significantly decreased compared to the control group. High expression of TFAM enhanced the proliferation ability of renal cancer cells， while low expression of TFAM inhibited the proliferation and invasion abilities of the cells. TFAM can target and regulate CDC25B and CCNE2， and it may promote renal cancer cell proliferation by activating CDC25B and CCNE2. Conclusion TFAM can promote the proliferation and invasion of renal cancer cells.
Key words： transcription factor A; mitochondrial; renal cancer cells; proliferation; invasion
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