lncRNA MIF-AS1调节miR-423-5p/PYCR1轴对前列腺癌细胞恶性生物学行为的影响
收稿日期: 2023-11-28
网络出版日期: 2024-09-30
基金资助
国家自然科学基金面上项目(81973822);四川省科技厅课题项目(2018JY0368)
Effect of lncRNA MIF⁃AS1 on the malignant biological behavior of prostate cancer cells by regulating the miR⁃423⁃5p/PYCR1 axis
Received date: 2023-11-28
Online published: 2024-09-30
目的 探究长链非编码RNA（lncRNA）巨噬细胞移动抑制因子反义RNA1（MIF-AS1）调节miR-423-5p/吡咯啉-5-羧酸还原酶1（PYCR1）轴对前列腺癌（PC）细胞恶性生物学行为的影响。 方法 体外培养PC3细胞，敲低MIF-AS1或下调miR-423-5p表达，检测PC患者肿瘤组织与癌旁组织和细胞中MIF-AS1、miR-423-5p和PYCR1 mRNA的表达；检测细胞增殖、凋亡、迁移与侵袭；Western blot检测PYCR1蛋白表达；验证miR-423-5p和MIF-AS1、PYCR1的关系。 结果 MIF-AS1、PYCR1 mRNA在肿瘤组织中呈高表达，miR-423-5p呈低表达。沉默MIF-AS1可抑制PC3细胞增殖、迁移、侵袭及PYCR1表达，上调miR-423-5p表达，诱导细胞凋亡（P < 0.05）；抑制miR-423-5p表达可逆转沉默MIF-AS1对PC3细胞恶性行为的抑制作用（P < 0.05）。MIF-AS1、PYCR1与miR-423-5p存在靶向调控关系。 结论 沉默MIF-AS1可能通过上调miR-423-5p来抑制PYCR1表达，抑制PC细胞的恶性行为。
杨剑波 , 邵继春 , 曾治军 , 赵涛 , 王兴 . lncRNA MIF-AS1调节miR-423-5p/PYCR1轴对前列腺癌细胞恶性生物学行为的影响[J]. 实用医学杂志, 2024 , 40(18) : 2544 -2549 . DOI: 10.3969/j.issn.1006-5725.2024.18.006
Objective To investigate the effect of long non-coding RNA （lncRNA） macrophage migration inhibitory factor antisense RNA1 （MIF-AS1） on the malignant biological behavior of prostate cancer （PC） cells by regulating the miR-423-5p/pyrroline-5-carboxylic acid reductase 1 （PYCR1） axis. Methods PC3 cells were cultured in vitro to knock down the expression of MIF-AS1 or down-regulate the expression of miR-423-5p. The expression of MIF-AS1， miR-423-5p and PYCR1 mRNA in tumor tissues and adjacent tissues and cells of PC patients were detected. The cell proliferation， apoptosis， migration， and invasion were detected and the expression of PYCR1 protein was detected by Western blot. The relationships between miR-423-5p， IF-AS1 and PYCR1 were verified. Results The MIF-AS1 and PYCR1 mRNA were observed to be highly expressed in the tumor tissues， while miR-423-5p was lowly expressed. Silenced MIF-AS1 inhibited the proliferation， migration and invasion of PC3 cells and up-regulated miR-423-5p induced cell apoptosis （P < 0.05）. Inhibition of miR-423-5p expression reversed the inhibitory effect of silencing MIF-AS1 on malignant behavior of PC3 cells （P < 0.05）. miR-423-5p was correlated with MIF-AS1 and PYCR1 by targeted regulation. Conclusion Silencing MIF-AS1 may inhibit the expression of PYCR1 by up-regulating miR-423-5p， thereby inhibiting the malignant behavior of PC cells.
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