目的 探究miR-4524a-3p在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMCs）及主要免疫细胞中的表达情况及其临床价值。 方法 收集SLE患者和健康对照的外周血，采用人外周血淋巴细胞分离液（Ficoll）及免疫磁珠细胞分选法分离PBMCs、CD3⁺T、CD19⁺B淋巴细胞及CD14⁺单核细胞；应用荧光定量PCR法检测miR-4524a-3p表达水平；采用Pearson线性相关分析评估miR-4524a-3p与SLE患者疾病活动度评分（SLEDAI）的关系；应用受试者工作特征曲线（ROC）评估miR-4524a-3p对SLE的诊断效能。 结果 与健康对照相比，miR-4524a-3p在SLE患者PBMCs（P < 0.05）、CD3⁺T细胞（P < 0.001）及CD14⁺单核细胞（P < 0.001）中表达显著降低，与SLEDAI评分呈负相关（r = -0.406，P < 0.01）；miR-4524a-3p在中度或重度活动期患者体内的表达水平显著低于轻度及非活动期患者（P < 0.01）；SLE狼疮性肾炎和关节受累组中，miR-4524a-3p表达水平低于无累及组（P < 0.01）；ROC曲线结果显示，miR-4524a-3p对SLE具有良好的诊断价值。 结论 miR-4524a-3p在SLE患者CD3⁺T和CD14⁺单核细胞中表达显著降低，与疾病活动度密切相关，对SLE具有一定的临床诊断价值。

Objective To analyze the expression of miR-4524a-3p in peripheral blood mononuclear cells （PBMCs） and major immune cell subsets of patients with systemic lupus erythematosus （SLE）， and evaluate its clinical application. Methods Peripheral blood samples were collected from SLE patients and healthy controls， followed by isolation of PBMCs， CD3⁺ T lymphocytes， CD19⁺ B lymphocytes， and CD14⁺ monocytes using human peripheral blood lymphocyte isolation solution （Ficoll） and immunomagnetic bead cell sorting. The expression level of miR-4524a-3p was quantified using fluorescence quantitative PCR. Pearson's linear correlation analysis was performed to assess the relationship between miR-4524a-3p expression and the disease activity score in SLE patients （SLEDAI）. Additionally， the diagnostic efficacy of miR-4524a-3p for SLE was evaluated by constructing a receiver operating characteristic curve （ROC）. Results The expression of miR-4524a-3p was significantly decreased in PBMCs （P < 0.05）， CD3⁺T cells （P < 0.001）， and CD14⁺ monocytes （P < 0.001） from patients with SLE compared to healthy controls. Furthermore， it exhibited a negative correlation with SLEDAI score （r = -0.406，P < 0.01）. Notably， the expression level of miR-4524a-3p was significantly lower in patients with moderate or severe active stage than those in mild and inactive stages （P < 0.01）. In addition， the SLE subgroup with lupus nephritis and joint involvement displayed lower levels of miR-4524a-3p compared to the subgroup without such manifestations （P < 0.01）. Importantly， ROC analysis demonstrated that miR-4524a-3p holds promising diagnostic value for SLE. Conclusion The expression of miR-4524a-3p was significantly diminished in CD3⁺ T cells and CD14⁺ monocytes derived from patients with SLE， exhibiting a close association with disease activity and demonstrating potential clinical diagnostic value for SLE.