目的 研究红景天苷预处理脂肪干细胞（ADSCs）促进糖尿病大鼠皮肤创面愈合的作用及机制。 方法 分离 ADSCs，分别用低糖培养基、高糖培养基、红景天苷联合高糖培养基处理，检测细胞的增殖、迁 移及角蛋白 19（CK19）、CD31、α⁃平滑肌肌动蛋白（α⁃SMA）的表达；制备糖尿病皮肤创面大鼠模型，移植 ADSCs 或红景天苷预处理的 ADSCs，观察创面愈合情况，检测创面中Ⅰ型胶原（Col⁃I）、Ⅲ型胶原（Col⁃Ⅲ） 及 CK⁃19、CD31、α⁃SMA 的表达。结果 高糖组 ADSCs 的 A490、迁移数目及 CK⁃19、CD31、α⁃SMA 的表达量均 低于对照组（P < 0.05），高糖+红景天苷组 ADSCs 的 A490、迁移数目及 CK⁃19、CD31、α⁃SMA 的表达量均高于 高糖组（P < 0.05）；ADSCs 组、红景天苷+ADSCs 组大鼠的创面愈合率及创面中 Col⁃Ⅰ、Col⁃Ⅲ、CK⁃19、 CD31、α⁃SMA 的表达量均增加（P < 0.05）且红景天苷+ADSCs 组的创面愈合率及创面中 Col⁃Ⅰ、Col⁃Ⅲ、 CK⁃19、CD31、α⁃SMA 的表达量均高于 ADSCs 组（P < 0.05）。结论 红景天苷预处理 ADSCs 能够促进糖尿 病大鼠皮肤创面的愈合，促进ADSCs 

Objective The study investigated the effect and mechanism of salidroside pretreated ADSCs on promoting skin wound healing of diabetic rats. Methods The ADSCs were isolated and treated with low glucose medium，high glucose medium，salidroside combined with high glucose medium respectively. Cell proliferation， migration and expression of cytokeratin 19（CK19），CD31，α⁃smooth muscle actin（α⁃SMA）in three groups were detected. The diabetic skin wounds rat model was prepared and transplanted with ADSCs or salidroside pretreated ADSCs. The wound healing was observed and the expressions of CoL ⁃Ⅰ，COL ⁃Ⅲ，CK ⁃19，CD31 and α⁃SMA were detected. Results The A490，migration numbers and the expression of CK⁃19，CD31，α⁃SMA of ADSCs in the high glucose group were lower than those in control group（P < 0.05）；the A490，migration numbers and the expression of CK⁃19，CD31，α⁃SMA of ADSCs in high glucose+salidroside group were obviously higher than those in high glucose group（P < 0.05）. The wound healing rate and the expression of CoL⁃Ⅰ，COL⁃Ⅲ，CK⁃19，CD31 and α⁃SMA in wound of the ADSCs group and salidroside+ADSCs group were significantly higher than model group （P < 0.05）and the wound healing rate and the expression of CoL ⁃Ⅰ，COL ⁃Ⅲ，CK ⁃19，CD31 and α⁃ SMA in wound of the salidroside+ADSCs group were notable higher than those in ADSCs group（P < 0.05）. Conclusion Salidroside pretreated ADSCs could promote the healing of diabetic skin wounds，and promote the proliferation，