目的 探讨白鲜碱对人前列腺癌细胞上皮⁃间充质转化的抑制作用及其机制。方法 培养 人前列腺癌 PC⁃3 细胞，用白鲜碱处理 PC⁃3 细胞，通过 MTS 检测白鲜碱的半抑制浓度 IC50及对 PC⁃3 细胞 增殖的时间依赖性。细胞侵袭能力的检测采用 Transwell 实验；E⁃cadherin、vimentin、β⁃catenin 和 Snail 蛋白 表达水平的检测采用 Western blot 实验；使用 Wnt 通道激活剂（LiCl）和白鲜碱联合作用 PC⁃3 细胞后，通过 Western blot 实验检测 Snail 和 E⁃cadherin 的 mRNA 及蛋白表达。结果 Transwell 实验结果表明，与对照组 相比，白鲜碱作用PC⁃3细胞后侵袭能力下降（P＜0.01）。PCR及Western blot的结果表明，白鲜碱作用PC⁃3细 胞后 E⁃cadherin 的 mRNA 及蛋白表达升高，vimentin 和 Snail 及细胞核中β⁃catenin mRNA 及蛋白的表达降低 （P＜0.01）。与白鲜碱组比较，白鲜碱与LiCl 联合作用PC⁃3细胞后Snail mRNA 及蛋白表达升高，E⁃cadherin mRNA 及蛋白表达降低（P＜0.01）。结论 白鲜碱可能通过抑制 Wnt/β⁃catenin/Snail 信号通路而抑制 PC⁃3 细胞上皮⁃

Objective To investigate the inhibitory effect of dictamnine onepithelial⁃mesenchymal transition of human prostate cancer cells and its mechanism. Methods PC⁃3 cells of human prostate cancer were cultured； PC⁃3 cells were treated with dictamnine，and the semi⁃inhibitory concentration IC50 of dictamnine and its time⁃ dependent effect on the proliferation of PC⁃3 cells were detected by MTS. The invasion ability of cells was detected by transwell assay. The expression levels of E ⁃cadherin，vimentin，β⁃catenin and snail were detected by Western blot. The protein expression of snail and E⁃cadherin was detected by Western blot assay after pc⁃3 cells were treated with Wnt channel activator LiCl and dictamnine. Results Transwell experiment showed that compared with that in the control group，the invasion ability of PC⁃3 cells was decreased after the treatment with dictamnine（P < 0.01）. PCR and western blot showed that the expression of E⁃cadherin mRNA and protein was increased，while the mRNA and protein expression of vimentin and snail and β⁃catenin in nucleus were decreased（P < 0.01）. Compared with that in dictamenine group，the expression of snail mRNA and protein was increased and the expression of E⁃cadherin mRNA and protein was decreased in PC⁃3 cells treated with dictamnine and LiCl（P < 0.01）. Conclusion Dictamenine may inhibit pc⁃3 cell epithelial⁃mesenchymal transformation by inhibiting the Wnt/ catenin/Snail signaling pathway.