收稿日期: 2023-07-29
网络出版日期: 2024-07-15
基金资助
湖北省卫生健康科研基金资助项目(WJ2021M054)
Application of leukocyte⁃and platelet⁃rich fibrin in dental pulp repair of rat
Received date: 2023-07-29
Online published: 2024-07-15
目的 探讨富白细胞-血小板纤维蛋白（leukocyte-and platelet-rich fibrin， L-PRF）作为盖髓剂对大鼠牙髓炎症调节的作用。 方法 脂多糖（LPS）构建牙髓炎大鼠模型，30只大鼠随机分成5组，即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-PRF + iRoot BP PLUS组。直接盖髓14 d后取标本行显微CT（Micro-CT）扫描、苏木精-伊红（HE）及Masson染色、定量实时聚合酶链式反应（qRT-PCR）检测，观察牙髓炎症及硬组织形成程度。 结果 Micro-CT示：LPS组未见硬组织形成，L-PRF组冠髓内可见斑块状钙化物，BP组及联合组在穿髓孔处见较完整的钙化桥。组织学染色示：LPS组炎症最重、无硬组织形成；联合组炎症最轻，硬组织形成最完整、连续，两组差异有统计学意义（P < 0.01）；BP组与联合组的炎症及硬组织形成程度均差异无统计学意义（P > 0.05）。qRT-PCR结果示：各实验组牙髓组织中TNF-α、IL-1β、IL-6 mRNA相对表达水平相较于LPS组均有所降低，但L-PRF组与LPS组的差异无统计学意义（P > 0.05）。 结论 L-PRF联合iRoot BP PLUS作为复合盖髓材料，可用于大鼠牙髓炎牙髓组织修复以及牙髓炎症调节。
关键词： 富白细胞-血小板纤维蛋白; 牙髓炎; 直接盖髓; 炎症反应; 修复性牙本质
王婷 , 何俐 . 富白细胞-血小板纤维蛋白在大鼠牙髓修复中的应用[J]. 实用医学杂志, 2024 , 40(14) : 1941 -1946 . DOI: 10.3969/j.issn.1006-5725.2024.14.007
Objective To explore the effect of leukocyte-and platelet-rich fibrin （L-PRF） as a capping agent in the modulation of inflammation in rat dental pulp. Methods The rat model of pulpitis was established by lipopolysaccharide（LPS）. A total of 30 Sprague-Dawley rats were randomly divided into five groups： Normal control group， Model group， iRoot BP PLUS group， L-PRF group， iRoot BP PLUS+ L-PRF group. Samples were collected on the 14th day after pulp capping. Micro-CT scans， HE and Masson staining，qRT-PCR assays were performed to observe pulp tissue inflammatory and hard tissue formation. Results Micro-CT results： no hard tissue formation was seen in the LPS group， plaque calculus was seen in the L-PRF group， and a more complete calcified bridge was seen at the perforation of the pulp in the BP and combined groups； HE and Masson staining results： the LPS group had the most severe inflammation and no hard tissue formation； the combined group had the least inflammation and the most complete and continuous hard tissue formation， and there was a statistical difference between the two groups（P < 0.01）； There was no statistical difference in the degree of inflammatory reaction and hard tissue formation between the BP group and the combined group （P > 0.05）；qRT-PCR results： the relative expression levels of TNF-α， IL-1β， and IL-6 mRNA in the pulp tissues of all experimental groups were reduced compared with those of the LPS group， but the differences between the L-PRF and LPS groups were not statistically significant （P > 0.05）. Conclusion The combination of L-PRF and iRoot BP PLUS as a composite pulp capping material can be used for the repair of dental pulp tissue and the regulation of inflammation in dental pulpitis in rats.
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