目的 建立血细胞处理仪（NGL-BBS）新程序制备洗涤混合血小板，与传统手工法比较对血小板生物活性的影响。 方法 用白膜法从无偿献血者捐献的全血中分离并制备混合浓缩血小板，分别采用血细胞处理仪（NGL-BBS）设定的新程序（观察组）和手工法（对照组）进行制备洗涤混合血小板，修改血细胞处理仪原洗涤红细胞程序参数建立新的洗涤血小板程序，具体参数如下：稀释量150 mL，洗涤量150 mL，稀释速度100 r/min，洗涤速度120 r/min。流式细胞术比较两组洗涤混合血小板的生物活化标志物CD62P和CD63的表达率，并使用血栓弹力图仪（TEG）测量和比较两组洗涤混合血小板的MA值。 结果 观察组洗涤混合血小板CD62P和CD63表达率均低于对照组（t = 4.11，P < 0.01；t = 10.78，P < 0.01）；观察组血小板的血栓弹力图MA值显著大于对照组（t = 6.67，P < 0.01）。 结论 本研究建立的血细胞处理仪（NGL-BBS）制备洗涤混合血小板的方法对生物活性的影响明显小于手工制备法。

Objective To establish a new program of blood cell processing apparatus（NGL-BBS） for the preparation of washed mixed plateletsand to study the effect on biological activity of platelets compared with traditional manual method. Methods Mixed concentrated platelets were separated and prepared from whole blood by white membrane method. Blood cell processing apparatus with new program set（experimental group） and manual method （control group） was used for thepreparation of washed mixed platelets. The expression rate of CD62P and CD63 in the two groups of washed mixed platelets was compared by flow-cytometry. Thrombus elastography （TEG） was used to measure and compare the MA value between the two groups. Results The expression rate of CD62P and CD63 in the experimental group was lower than that in the control group（t = 4.11，P < 0.01； t = 10.78，P < 0.01）. TheTEG MA valueof the experimental group was higher than that of the control group（t = 6.67，P < 0.01）. Conclusion The present study demonstrates that the use of NGL-BBS for the preparation of washed mixed platelets has a lesser impact on biological activity compared to manual preparation methods.