收稿日期: 2023-11-30
网络出版日期: 2024-05-15
基金资助
江苏省重点专科基金(苏卫办医政［2018］3号);徐州市科技计划项目(KC20121)
Expression and significance of miR⁃223⁃3p in serum of pregnant women with preterm premature rupture of membranes
Received date: 2023-11-30
Online published: 2024-05-15
目的 探讨miRNA-223-3p（miR-223-3p）在未足月胎膜早破（PPROM）孕妇血清中的表达水平及临床意义。 方法 选取2023年4~9月徐州医科大学附属徐州妇幼保健院行剖宫产术分娩的孕妇91例，其中PPROM孕妇60例及同期足月正常孕妇31例（正常组）作为观察对象。根据胎膜病理结果将观察组分为：未足月胎膜早破（PPROM）未合并HCA组（PPROM组，n = 37）；PPROM合并HCA组（PPROM+HCA组，n = 23）。收集入院治疗前孕妇血清样本，实时荧光定量（qRT）-PCR法检测血清中miR-223-3p的表达水平，酶联免疫吸附（ELISA）法检测血清IL-1β、IL-6、IL-8、TNF-α等炎症因子水平。 结果 PPROM+HCA组血清中miR-223-3p的表达水平较PPROM组、正常组显著增加（P < 0.05）。PPROM + HCA组血清中IL-1β、IL-6、IL-8、TNF-α的表达水平较PPROM组、正常组显著增加（P < 0.05）。PPROM + HCA组孕妇血清中miR-223-3p的表达水平与IL-1β、IL-6、IL-8、TNF-α表达水平呈正相关（r = 0.553、0.505、0438、0.656，P < 0.05）。 结论 miR-223-3p在PPROM+HCA孕妇血清中表达上调，与PPROM的炎症严重程度有关。
关键词： miR-223-3p; 胎膜早破; 绒毛膜羊膜炎; 炎症因子
胡玉莹 , 朱锦明 , 刘洁 , 潘亚文 , 张倩 , 冯锦秋 . 未足月胎膜早破孕妇血清中miR-223-3p的表达水平及临床意义[J]. 实用医学杂志, 2024 , 40(9) : 1275 -1279 . DOI: 10.3969/j.issn.1006-5725.2024.09.016
Objective Investigate the expression level and clinical significance of microRNA?223?3p （miR?223?3p） in the serum of pregnant women with preterm premature rupture of membranes （PPROM）. Methods A total of 91 pregnant women who underwent cesarean delivery at the Xuzhou Maternal and Child Health Hospital affiliated with Xuzhou Medical University between April and September 2023 were selected for the study. This included 60 cases of PPROM and 31 cases of term normal pregnant women as the research subjects. The study group was divided based on the pathological results of the membranes into： PPROM group without histological chorioamnionitis （HCA） （PPROM group， n = 37）， and PPROM group with HCA （PPROM+HCA group， n = 23）. Serum samples from pregnant women were collected before hospital treatment， and the expression level of miR?223?3p in the serum was detected by quantitative real?time PCR （qRT?PCR）. The levels of inflammatory factors such as IL?1β， IL?6， IL?8， and TNF?α in the serum were measured by enzyme?linked immunosorbent assay （ELISA）. Results The expression level of miR?223?3p in the serum of the PPROM+HCA group was significantly higher than that of the PPROM group and the normal group （P < 0.05）. The expression levels of IL?1β， IL?6， IL?8， and TNF?α in the serum of the PPROM+HCA group were also significantly higher than those of the PPROM group and the normal group （P < 0.05）. There was a positive correlation between the expression level of miR?223?3p in the serum of the PPROM+HCA group and the expression levels of IL?1β， IL?6， IL?8， and TNF?α （r = 0.553， 0.505， 0438， 0.656， P < 0.05）. Conclusion The upregulation of miR?223?3p in the serum of pregnant women with PPROM+HCA is associated with the severity of inflammation in PPROM.
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