收稿日期: 2023-04-24
网络出版日期: 2024-04-19
基金资助
河北省卫生健康委员会医学科学研究课题计划(20190583)
MAPK improves intestional obstruction in pregnancy through inhibiting TLR4/Myd88/NF⁃κB pathway
Received date: 2023-04-24
Online published: 2024-04-19
目的 分析MAPK通过调控TLR4/MyD88/NF-κB通路对妊娠期肠梗阻胃肠功能的影响。 方法 选取105只SPF级Wistar大鼠，雌性、雄性分别为70、35只，将所有大鼠同笼放置后，共有33只雌性大鼠妊娠成功，将妊娠成功的8只作为空白组，剩余25只雌性大鼠建立肠梗阻模型，最终建模成功24只，将其分为模型组、上调组、下调组各8只。 结果 与模型组相比，上调组MAPK表达量、VIP、GAS、TNF-α、IL-6水平、TLR4、Myd88、NF-κB mRNA表达量及相对表达量上升，MOT、IL-4、IL-10水平下降，下调组MAPK表达量、VIP、GAS、TNF-α、IL-6水平、TLR4、Myd88、NF-κB mRNA表达量及相对表达量下降，MOT、IL-4、IL-10水平上升（P < 0.05）。 结论 妊娠期肠梗阻大鼠经下调MAPK干预后胃肠功能改善，炎性反应减轻，其机制可能与TLR4/Myd88/NF-κB通路得到抑制有关。
高飞 , 李景 , 李洪健 . MAPK通过调控TLR4/Myd88/NF-κB通路对妊娠期肠梗阻胃肠功能的影响[J]. 实用医学杂志, 2024 , 40(8) : 1095 -1100 . DOI: 10.3969/j.issn.1006-5725.2024.08.013
Objective To investigate the effect of MAPK on intestinal obstruction during pregnancy. Methods A total of 105 SPF Wistar rats （female = 70 and male = 35） were selected. After placing all the rats in the same cage， 33 female rats were pregnant， and 8 rats were assigned to the control group and the other 25 rats were used to established bowel obstruction model. A total of 24 rats were successfully modelled， which were divided into model group（n = 8）， up-regulation group（n = 8）， and down-regulation group（n = 8）. Results Compared with the model group， MAPK expression， VIP， GAS， TNF-α， IL-6 levels， TLR4， Myd88， NF-κBmRNA were increased and MOT， IL-4， IL-10 levels were decreased in up-regulation group； MAPK expression， VIP， GAS， TNF-α， IL-6 levels， TLR4， Myd88， NF-κB mRNA were decreased and MOT， IL-4， IL-10 levels were increased in down-regulation group （P < 0.05）. Conclusion MAPK down-regulation reduced inflammatory response and improved bowel obstruction in pregnancy， and the mechanism may be related to the inhibition of TLR4/Myd88/NF-κB pathway.
| 1 | 蒋琴，蒋文军，刘文英，等. 急性肠梗阻患儿血清乳酸脱氢酶、碱性磷酸酶、白细胞与高敏C反应蛋白的表达及临床意义［J］. 实用医学杂志，2021，37（11）：1433-1436. doi:10.3969/j.issn.1006-5725.2021.11.012 |
| 2 | GEIST A C B， MOTTIN C C， DILLENBURG C F， et al. Intestinal obstruction following gastric bypass［J］. Obes Res Clin Pract， 2021，15（3）：291-292. doi:10.1016/j.orcp.2021.04.001 |
| 3 | ZHAO X Y， WANG X， LI C Q， et al. Intestinal obstruction in pregnancy with reverse rotation of the midgut： A case report［J］. World J Clin Cases， 2020，8（16）：3553-3559. doi:10.12998/wjcc.v8.i16.3553 |
| 4 | HSU P K， YANG P Y， SU W W， et al. Small bowel obstruction caused by infant compression during pregnancy［J］. Dig Liver Dis， 2020，52（11）：1370-1371. doi:10.1016/j.dld.2020.03.029 |
| 5 | 孟莹，刘宝清，李乔，等. 小承气汤合黄芪建中汤灌肠对术后早期炎性肠梗阻大鼠肠黏膜损伤及炎性因子的影响［J］. 中国中医急症， 2023，32（1）：51-54. doi:10.3969/j.issn.1004-745X.2023.01.011 |
| 6 | 何宏湖，陈红常，廖嘉家，等. METTL3通过调节MAPK和NF-κB信号通路参与脂多糖诱导小鼠小胶质细胞的细胞炎症反应［J］. 中国临床神经科学， 2021，29（2）：121-127. |
| 7 | LEE J H， KEDIA P， STAVROPOILOS S N， et al. AGA Clinical Practice Update on Endoscopic Management of Perforations in Gastrointestinal Tract： Expert Review［J］. Clin Gastroenterol Hepatol， 2021，19（11）：2252-2261. doi:10.1016/j.cgh.2021.06.045 |
| 8 | REN X， WANG Y， HE Z， et al. Effects of cefuroxime axetil combined with Xingpi Yanger granules on the serum gastrin， motilin， and somatostatin levels in children with upper respiratory tract infection accompanied by diarrhea： results of a randomized trial［J］. Transl Pediatr， 2021，10（8）：2106-2113. doi:10.21037/tp-21-314 |
| 9 | 周丽，王丹，潘小丽，等. 电针治疗肝胃不和型功能性消化不良的临床疗效及对血清胃泌素、胃动素水平的影响［J］. 实用医学杂志，2020，36（4）：538-542. doi:10.3969/j.issn.1006-5725.2020.04.024 |
| 10 | FURUTANI Y， HIRANUMA C， HSTTORI M， et al. A case of portal venous gas after obstructive transverse colon cancer surgery［J］. Surg Case Rep， 2019，5（1）：175. doi:10.1186/s40792-019-0729-z |
| 11 | SANCHEZ-RUIZ M， IURGU A M， KUSTER F， et al. CD8 T cell-Derived Perforin and TNF-α Are Crucial Mediators of Neuronal Destruction in Experimental Autoimmune Enteric Ganglionitis［J］. Am J Pathol， 2021，191（6）：1064-1076. doi:10.1016/j.ajpath.2021.02.021 |
| 12 | DOTLACIL V， BRONSKY J， HRADSKY O， et al. The Impact of Anti-Tumor Necrosis Factor Alpha Therapy on Postoperative Complications in Pediatric Crohn's Disease［J］. Eur J Pediatr Surg， 2020，30（1）：27-32. doi:10.1055/s-0039-1697909 |
| 13 | YE X， XU H， XU Y. Dexmedetomidine alleviates intestinal barrier dysfunction and inflammatory response in mice via suppressing TLR4/MyD88/NF-κB signaling in an experimental model of ulcerative colitis［J］. Folia Histochem Cytobiol， 2022，60（4）：311-322. doi:10.5603/fhc.a2022.0029 |
| 14 | LU L， LI W， SUN C， et al. Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway［J］. JPEN J Parenter Enteral Nutr， 2020，44（7）：1308-1317. doi:10.1002/jpen.1744 |
| 15 | JIN S， GUAN T， WANG S， et al. Dioscin Alleviates Cisplatin-Induced Mucositis in Rats by Modulating Gut Microbiota， Enhancing Intestinal Barrier Function and Attenuating TLR4/NF-κB Signaling Cascade［J］. Int J Mol Sci， 2022，23（8）：4431. doi:10.3390/ijms23084431 |
| 16 | LI S， CHEN Y， ZHANG Y， et al. Polyphenolic Extracts of Coffee Cherry Husks Alleviated Colitis-Induced Neural Inflammation via NF-κB Signaling Regulation and Gut Microbiota Modification［J］. J Agric Food Chem， 2022，70（21）：6467-6477. doi:10.1021/acs.jafc.2c02079 |
| 17 | 刘继攀，孙伟，宋默，等. 间充质干细胞外泌体通过抑制COX-2和NF-κB激活及p38 MAPK磷酸化缓解小鼠溃疡性结肠炎［J］. 中国免疫学杂志， 2021，37（14）：1688-1692，1700. doi:10.3969/j.issn.1000-484X.2021.14.005 |
/
| 〈 |
|
〉 |