目的 以Sortilin为切入点，探究慢性肾衰竭（CRF）血管钙化的机制，并探讨三七干预的影响，以期为临床降低CRF心血管事件提供有效的方法。 方法 将36只雄性SD大鼠随机分为正常组、模型组、三七低、中、高剂量组、骨化三醇组，每组6只。采用腺嘌呤联合高磷饲料喂养复制CRF血管钙化大鼠模型。检测主动脉钙盐沉积，大鼠血清Scr、BUN、TG、TC、P、Ca，主动脉TLRs、Sortilin蛋白及炎症因子水平。 结果 模型组大鼠肾纤维化明显，肾间质许多腺嘌呤结晶；大量钙盐沉积。各治疗组肾纤维化、钙盐沉积均有不同程度的减轻。与正常组比较，模型组大鼠血清BUN、Cr、P、TG、TC、IFN-γ、IL-6、IL-10、IL-17A含量明显升高（P < 0.01），Ca含量明显降低（P < 0.01）。与模型组比较，三七中、高剂量组和骨化三醇组大鼠血清BUN、Cr、P、TG、TC、IFN-γ、IL-6、IL-10、IL-17A含量明显降低（P < 0.01），Ca含量明显升高（P < 0.05或P < 0.01）。与正常组比较，模型组大鼠主动脉组织BMP2、RUNX2、Sortilin、TLR7、TLR9蛋白的表达显著升高（P < 0.01），SM22α蛋白的表达显著下降（P < 0.01）。与模型组相比，三七低、中、高剂量组和骨化三醇组BMP2、RUNX2、Sortilin、TLR7、TLR9蛋白表达下降显著（P < 0.01），SM22α蛋白表达显著上调（P < 0.05或P < 0.01），三七高剂量组和骨化三醇组效果更显著。 结论 三七可改善CRF血管钙化模型大鼠的肾纤维化、血管钙化，其机制可能与调控Sortilin、TLRs的生物学功能有关。

Objective Taking Sortilin as the entry point， this study aims to explore the mechanism of vascular calcification in chronic renal failure （CRF） and explore the influence of Sanqi on Sortilin， TLRs and vascular calcification， and to provide an effective method for clinical reduction of cardiovascular events in CRF. Methods Thirty-six male SD rats were randomly divided into normal group， model group， low-， medium- and high-dose Sanqi group and calcitriol group， with 6 rats in each. The replicative CRF vascular calcification rat model was fed with adenine combined with high phosphorus diet. Aortic calcium salt deposition， serum creatinine （Scr）， urea nitrogen （BUN）， blood calcium （Ca）， blood phosphorus （P）， total cholesterol （TC）， triglyceride （TG）， TLRs and Sortilin protein in aorta and inflammatory factors were detected. Results In the model group， renal fibrosis was obvious and many adenine crystals were found in renal interstitium. Large deposits of calcium salts were found. Renal fibrosis and calcium salt deposition were alleviated in different degrees in all treatment groups. Compared with those in the normal group， the level of BUN， Cr， P， TG， TC， IFN-γ， IL-6， IL-10 and IL-17A in the serum of the model group was ascended （P < 0.01）， while the level of Ca was descended （P < 0.01）. Compared with those in the model group， the level of BUN， Cr， P， TG， TC， IFN-γ， IL-6， IL-10 and IL-17A in the serum of rats in the Sanqi medium and high dose group and calcitriol group was significantly decreased （P < 0.01）， and the contents of Ca were significantly increased （P < 0.05 or P < 0.01）. Compared with those in the normal group， the protein expression of BMP2， RUNX2， Sortilin， TLR7 and TLR9 in aortic tissue of rats in the model group was elevated （P < 0.01）， while the protein expression of SM22α was declined （P < 0.01）. Compared with those in the model group， the protein expression of BMP2， RUNX2， Sortilin， TLR7， and TLR9 in the low-， medium-， and high-dose Sanqi group and calcitriol group was decreased significantly （P < 0.01）； the protein expression of SM22α was increased significantly （P < 0.05 or P < 0.01）， and the high-dose Sanqi group and calcitriol group had more significant effects. Conclusion Sanqi can improve renal fibrosis and vascular calcification in CRF model rats， and its mechanism may be related to the regulation of biological functions of Sortilin and TLRs.