收稿日期: 2023-06-17
网络出版日期: 2024-03-26
基金资助
国家自然科学基金项目(81500648);山西省基础研究计划项目(20210302123076)
Correlation between IL-17 gene polymorphism and type 2 diabetes mellitus in Han population in southeast Shanxi Province
Received date: 2023-06-17
Online published: 2024-03-26
目的 研究山西省东南地区汉族人群白细胞介素-17（IL-17）基因单核苷酸多态性及其与2型糖尿病（T2DM）的相关性。 方法 收集健康对照组和T2DM组受试者基本信息及临床资料，提取全血DNA，采用聚合酶链反应-高温连接酶反应对IL-17基因4个位点（rs2275913、rs3819024、rs4711998和rs8193036）进行多态性检测。 结果 两组IL-17基因4个SNP位点基因型和等位基因频率分布差异均无统计学意义（P > 0.05），在校正年龄、BMI、WC和血脂异常等因素后，两组研究对象在不同遗传模型下的基因型及等位基因频率分布差异亦无统计学意义（P > 0.05）。进一步分析显示，T2DM组rs2275913位点AA基因型FPG和HOMA-IR显著低于AG和GG基因型，三组间差异有统计学意义（P < 0.05）；而三组间BMI、FINS、HbA1c水平差异无统计学意义（P > 0.05）。此外，T2DM组rs3819024位点AA基因型WC、HOMA-IR显著高于GG基因型，差异有统计学意义（P < 0.05）。 结论 IL-17基因rs2275913、rs3819024、rs4711998、rs8193036多态性与山西省东南地区汉族人群T2DM可能无关，IL-17 rs2275913位点AA基因型、rs3819024位点GG基因型分别与山西东南地区汉族T2DM患者FPG和HOMA-IR、WC和HOMA-IR相关，可能是IL-17影响糖代谢的潜在基因型。
李燕 , 王琦 , 郑路 , 冯李慧 , 马丽 , 魏健 , 刘良姝 . 白细胞介素-17基因多态性与山西东南地区汉族人群2型糖尿病的相关性[J]. 实用医学杂志, 2024 , 40(5) : 695 -701 . DOI: 10.3969/j.issn.1006-5725.2024.05.018
Objective To determine the correlation between single-nucleotide polymorphisms of IL-17 with type 2 diabetes in Han population in southeastern Shanxi Provincein. Methods The basic information and related clinical data of the subjects were collected of normal healthy controls and T2DM. Whole blood DNA was extracted， and IL-17 polymorphisms （rs2275913， rs3819024， rs4711998 and rs8193036） were analyzed by using a polymerase chain reaction-high temperature ligase reaction. Results There was no significant difference in the genotype and allele frequency distribution of the four SNP loci of IL-17 gene between the two groups （P > 0.05）. IL-17 polymorphism was not linked with T2DM in multiple genetic models after controlling for age， BMI， WC， and dyslipidemia （P > 0.05）. Further analysis showed that the levels of AA genotype was substantially lower of FPG and HOMA-IR levels when compared with the AG and GG genotypes， and the differences among the three groups were statistically significant （P < 0.05）. However， BMI， FINS， HbA1c and HOMA-βwas no statistical significance among the three groups （P > 0.05）. Besides， WC and HOMA-IR of AA genotype （rs3819024） were notable higher than those of GG genotype in T2DM group（P < 0.05）. Conclusions The IL-17 gene polymorphisms rs2275913， rs3819024， rs4711998， and rs8193036 in the Han population of southeast Shanxi Province may not be associated with T2DM. In this region Han T2DM patients， AA genotype at rs2275913 of the IL-17 gene is associated to FPG and HOMA-IR， while GG genotype at rs3819024 is related to WC and HOMA-IR， which could be the potential genotype of IL-17 impacting glucose metabolism.
Key words： type 2 diabetes mellitus; interleukin-17; genetic polymorphism
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