收稿日期: 2023-06-21
网络出版日期: 2024-02-22
基金资助
云南省“兴滇英才支持计划-名医专项”(YNWR-MY-2020-044);云南省科学技术厅-昆医联合专项课题(202001AY070001-009);心血管疾病国家重点实验室开放课题(2019kfyf-05)
LncRNA SENCR targeted miR⁃206 regulates proliferation and apoptosis of human vascular smooth muscle cells of aortic dissection tissues
Received date: 2023-06-21
Online published: 2024-02-22
目的 探讨主动脉夹层患者夹层组织中长链非编码RNA SENCR的表达变化及其对人血管平滑肌细胞增殖、凋亡的影响及潜在的分子机制。 方法 HE染色检测夹层组织病理变化，FISH检测夹层组织及人血管平滑肌细胞（HVSMCs）中SENCR的表达，RT-qPCR检测组织中SENCR和miR-206的表达水平。pcDNA3.1-SENCR过表达质粒或空载体pcDNA3.1转染到HVSMCs。CCK-8和Annexin V/PI双染法流式凋亡实验分别检测HVSMCs的增殖和凋亡。双荧光素酶报告验证SENCR和miR?206的靶向关系。 结果 SENCR在HVSMCs中主要定位在胞质和胞核，与正常主动脉组织相比，主动脉夹层组织中SENCR表达下调（P < 0.01），miR?206表达上调（P < 0.01）。过表达SENCR后，HVSMCs细胞增殖能力明显降低（P < 0.01），细胞凋亡水平显著增加（P < 0.01）。SENCR靶向负调控miR-206。 结论 SENCR在主动脉夹层组织中表达下调，过表达SENCR可能通过靶向下调miR?206抑制HVSMCs细胞增殖，促进细胞凋亡。
马润伟 , 穆纯杰 , 桂雯婷 , 邓瑶 , 赵敏章 , 柳民 , 宋怡 . LncRNA SENCR靶向miR⁃206调控人主动脉夹层血管平滑肌细胞增殖和凋亡[J]. 实用医学杂志, 2024 , 40(3) : 302 -308 . DOI: 10.3969/j.issn.1006-5725.2024.03.004
Objective To investigate the expression of lncRNA SENCR in aortic dissection （AD） tissues of AD patients and its effect on and mechanism in the proliferation apoptosis of human vascular smooth muscle cells （HVSMCs）. Methods HE staining was done to detect the pathological changes of AD tissues. Fluorescence in situ hybridization （FISH） and RT-qPCR were used to determine the expression of SENCR in the AD tissue and HVSMCs and the expression of SENCR and miR?206 in the tissues， respectively. HVSMCs were cultured and transfected with pcDNA3.1-SENCR overexpression plasmids， or pcDNA3.1 blank plasmid. Then cell proliferation and apoptosis were detected by CCK-8 method and Annexin V/PI double staining flow cytometry assay， respectively. Double luciferase report verified the targeting relationship between SENCR and miR-206. Results SENCR was mainly located in the cytoplasm and nucleus of HVSMCs. Compared with the normal tissue， the expression of SENCR in the AD tissues was down?regulated （P < 0.01）， but the expression of miR-206 was up?regulated （P < 0.01）. Overexpressed SENCR decreased the cell proliferation of HVSMCs （P < 0.01）， but significantly increased the cell apoptosis of HVSMCs （P < 0.01）. SENCR could target and negatively regulate miR-206. Conclusion The expression of SENCR is down-regulated in AD tissues， and overexpressed SENCR may inhibit the proliferation and promote the apoptosis of HVSMCs by targeting down?regulated miR?206.
Key words： aortic dissection; SENCR; vascular smooth muscle cells; miR-206; proliferation; apoptosis
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