同种异体移植物炎性因子-1在糖尿病睾丸大鼠模型中的表达及意义
收稿日期: 2023-04-24
网络出版日期: 2024-01-24
基金资助
广西泌尿系统疾病临床医学研究中心(2020AC03006);广西科技基地和人才专项(2019AC17009)
Expression of allograft inflammatory factor⁃1 in the testicular model of diabetes mellitus rats
Received date: 2023-04-24
Online published: 2024-01-24
目的 探讨同种异体移植物炎性因子-1（AIF-1）在糖尿病（DM）睾丸大鼠模型中的表达及其意义。 方法 糖尿病睾丸（DMT）大鼠模型，随机分为糖尿病睾丸4周组（DMT4W组）、糖尿病睾丸8周组（DMT8W）、糖尿病睾丸12周组（DMT12W）。正常对照组（NC组）分为正常对照4周组（NC4W）、正常对照8周组（NC8W）、正常对照12周组（NC12W）。组织病理学检测各组睾丸形态学变化，免疫组织化学检测AIF-1蛋白表达，免疫荧光法观察AIF-1和NF-κB p65蛋白表达。 结果 组织病理学显示，与NC组相比，DMT组内睾丸内生精细胞数量，支持细胞、间质细胞、精子数明显减少。免疫组织化学结果显示，与NC组相比，DMT组睾丸内AIF-1蛋白表达显著增加（P < 0.05）。免疫荧光结果显示，与NC组相比，DMT各组AIF-1和NF-κB p65蛋白荧光强度显著增强。 结论 DMT组织AIF-1蛋白过表达，提示其在DMT病变过程可能起重要作用，有望成为治疗新靶点和诊断标记物。
关键词： 同种异体移植物炎性因子-1; 糖尿病睾丸; 大鼠; 模型; 意义
李德朝 , 张明津 , 蓝一笔 , 马春雷 , 付伟金 . 同种异体移植物炎性因子-1在糖尿病睾丸大鼠模型中的表达及意义[J]. 实用医学杂志, 2024 , 40(1) : 65 -71 . DOI: 10.3969/j.issn.1006-5725.2024.01.012
Objective To investigate the expression of allograft inflammatory factor-1 （AIF-1） in the testicular model of diabetes mellitus（DM） rats as well as its significance. Methods The rat model of DM testis （DMT） was established， which were randomly divided into the DM testis 4-week group （DMT4W）， DM testis s 8-week group （DMT8W） and the DM testis 12-week group （DMT12W）. The normal control group （NC group） was randomly divided into three subgroups： NC 4-week （NC4W）， NC 8-week （NC8W） and NC 12-week （NC12W）. The morphologic changes of testis in the different groups was detected by histopathology. The expression of AIF-1 protein was detected by immunohistochemistry. The expression of AIF-1 and NF-κB p65 protein was observed by immunofluorescence. Results The histopathological results suggested that the numbers of spermatogenic cells， sertoli cells， interstitial cells and sperms in the DMT group were significantly decreased， as compared with the NC group. The immunohistochemistry results showed that the expression of AIF-1 protein was significantly increased in the DMT group， as compared with the NC group （P < 0.05）. The intensity of AIF-1 and NF-κB p65 in the DMT group was significantly increased by immunofluorescence， as compared with the NC group. Conclusion The over expression of AIF-1 protein in DMT tissue suggests that it may play an important role in the pathological process of DMT and may become a new therapeutic target and diagnostic marker in the future.
Key words： allograft inflammatory factor-1; diabetes mellitus testis; rat; model; significance
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