目的 探讨辛伐他汀联合机械牵拉对骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）成骨分化的影响及 Wnt/β⁃catenin 通路在其中的作用。方法 实验分为对照组、辛伐他汀组、 机械牵拉组及辛伐他汀+机械牵拉组，利用碱性磷酸酶（ALP）活性及 RT⁃PCR 检测各组 BMSCs 的 ALP 活性 及 ALP、OCN、Col 1a、RUNX2 基因表达，利用 RT⁃PCR 及 Western blot 检测β⁃catenin 通路相关基因和蛋白的 表达，并探讨 DKK1 处理后辛伐他汀联合机械牵拉对成骨分化的影响。结果 与对照组比，辛伐他汀、机械牵拉及辛伐他汀+机械牵拉均能增加 ALP 活性及成骨基因表达，并能增加β⁃catenin 基因和蛋白表达，且 以辛伐他汀+机械牵拉组升高最明显；使用 Wnt/β⁃catenin 信号通路抑制剂 DKK1 能减弱辛伐他汀+机械牵 拉组促成骨分化效果。结论 辛伐他汀及机械牵拉均促进 BMSCs 成骨分化，且辛伐他汀协同机械牵拉通 过激活Wnt/β⁃catenin 通路促进BMSCs 

Objective To investigate the effect of simvastatin combined with mechanical stretch on osteogenic differentiation of bone marrow mesenchymal stem cells（BMSCs）and explore its mechanisms. Methods simvastatin group，mechanical stretch group and simvastatin+mechanical stretch group in the study. ALP activity and RT ⁃ PCR were used to investigate the effect of simvastatin，mechanical stretch and simvastatin + mechanical stretch on osteogenic differentiation of BMSCs and RT⁃PCR and WB were used to investigate the expression of β ⁃catenin in each group. Results The level of osteogenic relative genes was up⁃regulated and the expression of β ⁃catenin protein was increased in each group when compared with those in the control group，especially in simvastatin+mechanical stretch group. DKK1 partly eliminated osteogenic differentiation of BMSCs in simvastatin+ mechanical stretch group. Conclusion Both simvastatin and mechanical stretch promote the osteogenic differentia⁃ tion of BMSCs. Simvastatin promotes the osteogenic differentiation of BMSCs induced by mechanical stretch via