Objective To study the effect of propofol regulating HIF-1α signaling pathway through miR182-5p on biological activity in placental trophoblast cells induced by Hypoxia. Methods Hypoxia-induced in vitro cultured HTR-8/SVneo cells were used in this study. Cells were treated with propofol. CCK-8 was used to detect cell viability； Transwell to determine cell migration and invasion， and TUNEL to detect apoptosis. The relative mRNA expression level of miR-182-5p and HIF-1α was measured by qRT-PCR. Western blot was used to detect the expression level of related pathway proteins. Results Compared with the control group， the hypoxic group showed reduced cell migration and invasion ability， increased apoptosis rate （P < 0.05）， reduced miR-182-5p and MMP-9 expression level， and increased HIF-1α and VEGF expression level （P < 0.05）. Compared with the hypoxic group， propofol intervention restored cell migration and invasion ability， decreased apoptosis rate （P < 0.05）， increased miR-182-5p ， MMP-9 expression level， and decreased HIF-1α and VEGF expression level （P < 0.05）. Transfection with inhibitors reversed the therapeutic effect of propofol. Conclusion Propofol ameliorates hypoxia-induced HTR-8/SVneo cytotoxicity by regulating the miR-182-5p/HIF-1α axis.