目的 探究单纯烟草烟雾慢性暴露诱导的慢性阻塞性肺疾病（chronic obstructive pulmonary disease， COPD）小鼠模型能否合并发生抑郁。方法 SPF 级小鼠分为对照组（CTL 组）和烟雾暴露组 （CS组）。将CS组小鼠连续24周暴露在烟草烟雾中，最后一次烟熏24 h后进行旷场测试、悬尾测试，再进行肺功能检测。ELISA检测支气管肺泡灌洗液（BALF）上清中炎症因子白细胞介素-6（IL-6）、角质形成细胞衍生趋化因子（KC）的水平变化，以及血清中5-羟色胺（5-HT）的含量变化。H&E染色和尼氏染色观察小鼠肺组织、脑 组织的病理学变化。结果 与 CTL 组相比， CS 组小鼠体质量增长缓慢（P < 0.001），肺功能检测 FRC、FVC 显著升高（P < 0.001），FEV20/FVC 和FEV50/FVC 显著降低（P < 0.05）；CS组小鼠的BALF 中IL-6、KC的表达水平升高（P < 0.01），血清中5-HT水平明显降低（P < 0.001）；旷场实验中CS组小鼠的运动总路程、进入中央区次数、中央区滞留时间均降低（P < 0.05），悬尾测试中CS组小鼠的静止不动时间增加（P < 0.05）；H&E染色显示CS组的肺组织平均肺泡截距显著增加（P < 0.001），尼氏染色显示CS组小鼠皮质（P < 0.001）和海马区 （P < 0.01）受损的神经元数量显著增加。结论 单纯烟草烟雾慢性暴露诱导的COPD小鼠模型可合并抑郁发生。 

Objective To explore whether depression can occur in COPD mouse model induced by chronic exposure to tobacco smoke alone. Methods SPF grade mice were divided into control group（CLT group） and smoke exposure group（CS group）. Mice in CS group were exposed to tobacco smoke for 24 weeks. Open field test and tail suspension test were performed 24 hours after the last smoking，and lung function test was performed after the test. The levels of IL­6 and KC in BALF supernatant were detected by ELISA， as well as the serum levels of 5­HT. H&E staining and Nissl staining were used to observe the pathological changes of lung and brain tissues of mice. Results Compared with CTL group，the body weight of mice in CS group grew slowly （P < 0.001）， FRC and FVC were significantly increased （P < 0.001），and FEV20/FVC and FEV50/FVC were significantly decreased （P < 0.05）. The expression levels of proinflammatory cytokines IL­6 and KC in BALF were increased （P < 0.01），and the content level of 5­HT in serum was significantly decreased （P < 0.001）. The total movement distance， the number of times into the central area and the retention time in the central area of the CS group were significantly decreased in the open field test （P < 0.05）， and the immobility time of the CS group was increased in the tail suspension test （P < 0.05）. H&E staining showed that the mean alveolar intercept of lung tissue in the CS group was significantly increased （P < 0.001）， and Nissl staining showed that the number of damaged neurons in the cortex （P < 0.001） and hippocampus （P < 0.01） in the CS group was significantly increased. Conclusion Chronic exposure to tobacco smoke alone induced COPD mouse models with depression.