目的 在成功制备万古霉素/PLGA/TCP 的多孔复合材料的基础上，建立羊股骨感染性骨缺 损模型，将复合支架植入骨缺损，观察其修复感染性骨缺损的效果。方法 建立苏博美利奴羊右侧股骨 感染性骨缺损模型，随机分为三组，实验组 1（3D 多孔支架组）植入万古霉素/PLGA/TCP多孔复合材料，实验组 2（骨水泥珠链组）植入含有万古霉素的聚甲基丙烯酸甲酯（PMMA）珠链，对照组 1（对照组）不植入任何材料，为单纯的骨缺损组，术后各时相点抽血测量羊血中白细胞数目、血沉和 C 反应蛋白变化；局部活 组织检查进行万古霉素含量及活性测定；二次手术后 12 周取材，行 X 线片检查及 HE 染色组织学分析，观 察支架的降解及骨生长情况。结果 所有实验动物存活。3D 多孔支架组与骨水泥珠链组都具有良好的 抗感染效果，手术后 4、8、12 周的血沉和 CRP 值明显下降，与对照组相比，差异有统计学意义（P < 0.05）。 影像学及组织学结果表明，与对照组相比，3D 多孔支架组骨缺损范围明显缩小（P < 0.01），可见更多的骨 长入（P < 0.05），骨缺损局部炎性细胞浸润不明显；与对照组相比，骨水泥珠链组炎性反应轻微，相反对照 组局部组织可见大量炎性细胞浸润，炎性反应明显。结论 采用生物打印技术制备的万古霉素/PLGA/ TCP 的多孔复合材料不仅可以掺加抗感染的药物 ，还可以根据骨缺损的形状定制植入材料，有望实现在 有效抗感染的同时，促进骨缺损的修复。

Objective Based on the success in preparation of vancomycin⁃incorporated PLGA/TCP scaf⁃ fold，its effect of repair infected femur defects in goats was evaluated. Methods A goat model of infected femur defects was established and the goats were randomly assigned to three groups. The experimental group 1 was implanted with vancomycin⁃incorporated PLGA/TCP scaffold，the control group 1 was implanted with antibiotic⁃PMMA（poly⁃ methyl methacrylate）and the control group 2 was served as a blank control group. White blood cells（WBC），erythro⁃ cyte sedimentation rate（ESR）and C⁃reactive protein（CRP）were detected at different time points. The content and antibacterial activity of vancomycin were detected in local tissues. X ⁃ ray and hematoxylin ⁃eosin（HE）stain were performed to assess biodegradation of the scaffold and bone reconstruction. Results All of the animals survived. The experimental group 1 and the control group 1 had better anti ⁃infective effect. As compared with the blank control group，ESR and CRP were significantly lower in the other two groups at weeks 4，8 and 12 after operation（P < 0.05）. Besides，the experimental group 1 showed more biodegradation and bone reconstruction than the control group （P < 0.05）. As compared with the control group，the degree of inflammatory reaction was less in the experimental group 1 and the control group 1. Conclusions The composite vancomycin⁃incorporated PLGA/TCP porous scaffold can not only be added anti⁃infective drugs，but also customize implant materials according to the shape of femur defects. The efficacy of anti⁃infection is hopefully achieved，