南华大学衡阳医学院附属第一医院 1 康复医学中心，2 康复医学科，3 康复医学实验室（湖南衡阳421001）； 4 衡阳市第一人民医院呼吸内科（湖南衡阳421001）
网络出版日期: 2023-01-25
基金资助
国家自然科学基金面上项目（编号：81973917）；国家自然科学基金青年项目（编号：82105022）；湖南省自然科学基金（编号：2021JJ40507）；湖南省卫健委项目（编号：202203104147）；南华大学校级重点项目（编号：USCKF201902K02）
Study on the mechanism of P53/P21 pathway in electroacupuncture inhibiting osteoblast aging in osteopo⁃ rotic rats
The First Affiliatcd Hospital，Department of Rehabilitation，Hengyang Medical School，Uni⁃ versity of South China，Hengyang 421001，China；*The First Affiliated Hospital，Rehabilitation Medicine Center， Hengyang Medical School，University of South China，Hengyang 421001，China；*The First Affiliated Hospital， Rehabilitation Laboratory，Hengyang Medical School，University of South China，Hengyang 421001，China
Online published: 2023-01-25
目的 探讨电针对老年大鼠骨质疏松及衰老细胞通路 P53/P21 的影响。方法 27 月龄雄性 大鼠随机分为老年组和电针治疗组（n = 8），电针治疗组接受电针干预（每周 5 次，共 8 周），6 月龄雄 性大鼠设为青年组。8 周后处死大鼠，ELISA 检测血清Ⅰ型胶原 C 末端肽（CTX⁃I）、Ⅰ型胶原羧基端前肽 （PICP）水平；Micro⁃CT 扫描骨组织；检测 P53、P21 等 mRNA 及蛋白表达水平。结果 老年组 CTX⁃I 较青 年组增加，PICP 较青年组降低（P < 0.01）；电针可降低老年大鼠 CTX⁃I，增高 PICP（P < 0.01）；Micro⁃CT 显 示相对青年组，老年组大鼠骨小梁分布紊乱、稀疏，BMD、BV/TV、Tb.N 等下降（P < 0.01），Tb.Sp 增高 （P < 0.01）。电针可改善骨小梁紊乱分布，增加 BMD、BV/TV、Tb.N（P < 0.05），降低 Tb.Sp（P < 0.01）。 相对于青年组，老年组大鼠 P53、P21 mRNA 及蛋白表达量增高（P < 0.01）。电针能下调老年大鼠 P53、 P21 mRNA 及蛋白表达量（P < 0.01）。结论 电针可能通过抑制 P53/P21 信号通路来缓解成骨细胞衰 老、增加成骨细胞活性，并以此来促进骨形成，改善骨质疏松。
关键词：
骨质疏松
钟培瑞, 何晓艳 廖瑛, 孙光华, 刘静, 周君, 李书枝, 刘媛, 屈萌艰, . P53/P21通路在电针抑制骨质疏松大鼠模型 成骨细胞衰老中的机制[J]. 实用医学杂志, 2023 , 39(2) : 192 -197 . DOI: 10.3969/j.issn.1006⁃5725.2023.02.012
Objective The research aimed to explore the effects of Electroacupuncture on osteoporosis and P53/P21 signal pathway in elderly rats. Methods Twenty⁃seven⁃month⁃old male SD rats were randomly divided into two groups：elderly group（n = 8），electroacupuncture treatment group（n = 8）. Six⁃month⁃old male SD rats were young group（n = 8）. The treatment group was treated with electroacupuncture intervention five times a week for a total of 8 weeks. All rats in each group were sacrificed at 8 weeks. The serum bone metabolism indexes（CTX⁃I， PICP）were detected by using ELISA system；Micro⁃CT scanner were performed to evaluate the microstructure of bone tissue；The expression levels of P53/P21 mRNA and protein were detected by RT⁃PCR and Western blot. Results The levels of serum CTX⁃I in elderly group were higher than the young group（P < 0.01），while PICP was lower than that in the young group（P < 0.01）. Electroacupuncture caused decrease in CTX⁃I and increase in PICP compared to the elderly group（P < 0.01）. The BMD，BV/TV，Tb.N of bone tissue in elderly group were significantly lower contrast with young group（P < 0.01），while the Tb.Sp in elderly group was significantly higher compared with young group（P < 0.01）. Compared with the elderly group，Electroacupuncture caused increase in BMD，BV/TV，Tb.N（P < 0.05）and decrease in Tb.Sp（P < 0.01）. The expressions of P53/P21 mRNA and protein in the elderly group were significantly higher than the young group（P < 0.01）. Compared with the elderly group，the expressions of P53/P21 mRNA and protein in the electroacupuncture treatment group were significantly reduced （P < 0.01）. Conclusion Electroacupuncture can effectively improve osteoporosis in elderly rats.The mechanisms may be to alleviate osteoblast aging and increase osteoblast activity by inhibiting P53/P21 signal pathway，so as to promote bone formation and reduce bone loss.
Key words：
osteoporosis
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