目的 探讨 circ_0005276 靶向 miR⁃557 对类风湿关节炎滑膜成纤维细胞（RA⁃FLS）增殖、 迁移和侵袭的影响。方法 RT⁃qPCR 检测 circ_0005276 和 miR⁃557 在 CIA 大鼠滑膜组织中的表达。将 si⁃circ_0005276、si⁃NC、pcDNA、pcDNA⁃circ_0005276、miR⁃NC、miR⁃557模拟物、si⁃circ_0005276+anti⁃miR⁃NC、 si⁃circ_0005276+anti⁃miR⁃557 分别转染 RA⁃FLS，CCK⁃8、划痕愈合、Transwell 实验用于检测 RA⁃FLS 的活力、 划痕愈合率以及侵袭数。双荧光素酶报告验证 circ_0005276 和 miR⁃557 的靶向关系。结果 与正常组滑 膜组织比较，CIA 大鼠滑膜组织中 circ_0005276 表达上调（P < 0.05），miR⁃557 表达下调（P < 0.05）。干扰 circ_0005276 后 RA⁃FLS 的光密度（OD）值、划痕愈合率、侵袭数显著降低（P < 0.05），miR⁃557 表达升高 （P < 0.05）。过表达circ_0005276后RA⁃FLS中miR⁃557表达显著降低（P < 0.05）。过表达miR⁃557后RA⁃FLS 的 OD 值、划痕愈合率、侵袭数显著降低（P < 0.05）。下调 miR⁃557 表达显著减弱了干扰 circ_0005276 表达 对 RA⁃FLS 的 OD 值、划痕愈合率和侵袭数的影响（P < 0.05）。结论 干扰 circ_0005276 可通过靶向促进 miR⁃557表达，从而抑制RA⁃FLS 增殖、迁移和侵袭。

Objective To investigate the effect of circ_0005276 targeted miR⁃557 on the proliferation， migration and invasion of rheumatoid arthritis synovial fibroblasts（RA⁃FLS）. Methods RT⁃qPCR was used to detect the expression of circ_0005276 and miR⁃557 in synovium of CIA rats. Si⁃circ_0005276，si⁃NC，pcDNA， pcDNA⁃circ_0005276，miR⁃NC，miR⁃557 mimic，si⁃circ_0005276+anti⁃miR⁃NC and si⁃circ_0005276+anti⁃miR⁃ 557 were transfected into RA⁃FLS，CCK⁃8，scratch healing and Transwell assay to detect the activity，scratch heal⁃ ing rate and invasion number of RA⁃FLS. Double luciferase report verified the targeting relationship between circ_ 0005276 and miR⁃557. Results Compared with the normal synovial tissue，the expression of circ_0005276 in synovial tissue of CIA rats was up⁃regulated（P < 0.05）and the expression of miR⁃557 was down⁃regulated（P < 0.05）. After interfering with circ_0005276，the optical density（OD），scratch healing rate and invasion number of RA⁃ FLS decreased significantly（P < 0.05），while the expression of miR⁃557 increased（P < 0.05）. The expression of miR⁃557 in RA⁃FLS was significantly decreased after overexpression of circ_0005276（P < 0.05）. The OD value， scratch healing rate and invasion number of RA ⁃FLS decreased significantly after overexpression of miR ⁃ 557 （P < 0.05）. Down⁃regulation of miR⁃557 expression significantly weakened the effect of interfering circ_0005276 expression on the OD value，scratch healing rate and invasion number of RA⁃FLS（P < 0.05）. Conclusion Inter⁃ ference with circ_0005276 could promote the expression of miR⁃557 by targeting，thus inhibit the proliferation，mi⁃ gration，and invasion of RA⁃FLS.