目的 探讨低温缺血再灌注心律失常大鼠心室肌电传导变化及其可能机制。方法 选择清 洁级2～3月龄健康雄性SD大鼠，制备离体心脏灌注模型16个，随机分为两组（n = 8），正常对照组（C组）： 持续灌注 37 ℃ K⁃H 液 120 min；低温缺血再灌注组（IR 组）：持续灌注 37 ℃ K⁃H 液 30 min 后停止灌注 60 min，随后再灌注 30 min。分别在持续灌注 15 min（T0）、持续灌注 30 min（T1）、再灌注 15 min（T2）、再灌注 30 min（T3）时点采集心率（HR）、传导速度（CV）和电传导图并记录心律失常情况；通过 Western blot 和免 疫组化检测再灌注后左心室前壁组织应急诱导磷蛋白 1（STIP1）、连接蛋白 43（Cx43）蛋白的表达和分 布。结果 IR 组：在再灌注期间，有 7 例发生心律失常；在 T2、T3时点与 T0和 T1比较，HR、CV 明显降低 （P < 0.05）且传导方向呈发散改变。与 C 组比较，IR 组心肌组织中 STIP1、Cx43 蛋白表达均明显降低（P < 0.05），IR 组 Cx43 蛋白着色的颗粒减少，有偏侧化现象。结论 STIP1 可能通过调节 Cx43 表达影响低温 缺血再灌注后心室肌电传导。

Objective To investigate the changes of ventricular myoelectric conduction and its possible mechanism in hypothermic ischemia⁃reperfusion arrhythmia rats. Methods Healthy male SD rats aged 2 ~ 3 months were selected to prepare isolated heart perfusion models（n = 16）. The rat models were randomly divided into normal control group（C group，continuous perfusion of 37 ℃ K⁃H solution for 120 min）and hypothermic ischemia⁃reper⁃ fusion group（IR group，continuous perfusion of 37 ℃ K⁃H solution for 30 min，reperfusion stopped for 60 min， and then reperfusion for 30 min），with 8 rats in each group. Heart rate（HR），conduction velocity（CV）and elec⁃ trical conduction chart at the timepoint of continuous perfusion for 15 min（T0），continuous perfusion for 30 min （T1），reperfusion for 15 min（T2）and reperfusion for 30 min（T3）were collected，and the arrhythmia was recorded. The expression and distribution of STIP1 and Cx43 in the anterior wall of left ventricle after reperfusion were detected by western blot and immunohistochemistry. Results IR group had 7 cases of arrhythmia during reperfusion. Anlayis on IR group showed，HR and CV were decreased significantly at T2 and T3 when compared with T0 and T1（all P < 0.05）and the conduction direction showed divergent change. The expression of STIP1 and Cx43 protein in myocar⁃ dial tissue of IR group were apparently decreased when compared with C group（both P < 0.05），and Cx43 positive stained particle numbers in IR group were also decreased，in which the phenomenon of lateralization was observed. Conclusions STIP1 may affect ventricular myoelectric conduction after hypothermic ischemia⁃reperfusion by regu⁃ lating the expression of Cx43.