目的 探索长链非编码 RNA LINP1 对电离辐射下非小细胞肺癌 DNA 损伤效应的影响，为非小细胞肺癌的肿瘤治疗提供相关的新思路。方法 使用 siRNA 在非小细胞肺癌细胞系 H1299 细胞 中敲减 LINP1，8.0 Gy 电离辐射照射后，采用 CCK⁃8 增殖实验和克隆形成实验检测肺癌细胞的增殖和 克隆形成能力；照射后 4 h 采用彗星电泳和 Western blot 检测 H1299 细胞中 DNA 损伤效应。结果 电离 辐射照射后，H1299 细胞中 LINP1 明显上调（P < 0.001）。与对照相比，8.0 Gy 电离辐射照射后其增殖 和克隆形成能力显著下降（P < 0.001），DNA 损伤明显增加（P < 0.001）。沉默 LINP1 后，在电离辐射条件 下，敲减组与对照相比增殖能力、克隆形成能力明显下降（P < 0.01），DNA 损伤明显增加（P < 0.001）。 结论 LINP1 可抑制电离辐射下非小细胞肺癌细胞增殖能力的下降和 DNA 损伤效应从而增强电离辐 射抗性。

Objective To explore the effect of long non⁃coding RNA LINP1 on ionizing radiation⁃induced DNA damage in non⁃small cell lung cancer（NSCLC）and provide theoretical basis for novel combination treatment option. Methods H1299 NSCLC cells were transfected with siRNAs.CCK ⁃ 8 assays and colony formation assays were used to check for H1299 cell growth after 8.0 Gy of ionizing radiation.To find the DNA damage in H1299 cells，Western blot and comet assay were used. Results After receiving ionizing radiation（IR），LINP1 was sig⁃ nificantly（P < 0.001）upregulated in H1299 cells. Compared with the control，the proliferation capacity and clon⁃ al formation ability decreased（P < 0.001），while the DNA damage（P < 0.001）was increased significantly（P < 0.001）. Under 8.0 Gy IR treatment，LINP1 knockdown significantly reduced H1299 cell proliferation and clonal formation ability（P < 0.01），as well as DNA damage repair ability（P < 0.001）. Conclusions LINP1 inhibits proliferation and increases IR⁃induced DNA damage in NSCLC cells，while also promoting radioresistance.