目的 探讨右美托咪定对宫颈癌细胞热休克蛋白 90（HSP90）/细胞外信号调节激酶（ERK） 通路及顺铂敏感性的影响。方法 体外培养人宫颈癌 HeLa 细胞，分为对照组、顺铂组、低浓度组和高浓 度组。蛋白印迹（Western blot）法检测各组 HeLa 细胞中 HSP90、ERK、p⁃ERK、DNA 甲基转移酶 1（DNMT1）、 DNA 聚合酶 δ 催化亚基（POLD1）、增殖细胞核抗原（PCNA）、Bcl⁃2 相关 X 蛋白（Bax）蛋白表达情况；CCK⁃8 法检测各组 HeLa 细胞增殖情况；流式细胞仪检测各组 HeLa 细胞凋亡情况。结果 与对照组相比，顺铂组 HeLa 细胞中 HSP90、p⁃ERK/ERK、DNMT1、POLD1、PCNA 蛋白水平、OD 值降低（P < 0.05），而凋亡率、Bax 蛋 白水平升高（P < 0.05）；随着右美托咪定的添加及浓度的升高，HeLa 细胞中 HSP90、p⁃ERK/ERK、DNMT1、 POLD1、PCNA 蛋白水平、OD 值依次降低（P < 0.05），而凋亡率、Bax 蛋白水平依次升高（P < 0.05）。结论 右美托咪定可抑制宫颈癌HeLa 细胞中HSP90/ERK 通路，增强HeLa 细胞对顺铂的敏感性。

Objective To investigate the effects of dexmedetomidine on heat shock protein 90（HSP90）/ extracellular signal regulated kinase（ERK）pathway and cisplatin sensitivity in cervical cancer cells. Methods Human cervical cancer HeLa cells were cultured in vitro and divided into control group，cisplatin group，low concentration group and high concentration group. Western blot was used to detect the expressions of HSP90， ERK，p⁃ERK，DNA methyltransferases1（DNMT1），DNA polymerase delta catalytic subunit gene 1（POLD1）， proliferating cell nuclear antigen（PCNA），and Bcl⁃2 associated X protein（Bax）protein in HeLa cells；the prolif⁃ eration of HeLa cells was detected by CCK⁃8 method；and the apoptosis of HeLa cells was detected by flow cytome⁃ try. Results Compared with those in the control group，the protein expression levels of HSP90，p ⁃ERK/ERK， DNMT1，POLD1，PCNA and OD value of HeLa cells in cisplatin group were significantly lower（P < 0.05），while the apoptosis rate and Bax protein expression level were significantly higher（P < 0.05）；with the addition and increase of dexmedetomidine，the protein expression levels of HSP90，p ⁃ ERK/ERK，DNMT1，POLD1，PCNA and OD value of HeLa cells decreased in turn（P < 0.05）while the apoptosis rate and Bax protein expression level increased in turn（P < 0.05）. Conclusion Dexmedetomidine can inhibit the HSP90/ERK pathway in cervical cancer HeLa cells and enhance the sensitivity of HeLa cells to cisplatin.