目的 研究宫颈鳞癌患者手术前后的三个不同时期以及与健康对照组之间的血清差异蛋 白质。方法 采用 SELDI⁃TOF⁃MS 技术结合弱阳离子结合芯片（CM10），对宫颈鳞癌术前（A1 组）、术后 7 ~ 10 d（A2 组）、术后 30 ~ 40 d（A3 组）及对照组的血清蛋白质指纹图谱进行动态分析，比较不同时期蛋白 质图谱变化，筛选出有差异的血清蛋白质。结果 共得出两组血清差异蛋白质，第一组为宫颈鳞癌与对 照组比较后的差异蛋白质，共 16 个，其中的 12 个差异蛋白质在 A1 组与 A2 组比较中差异有统计学意义； 这 16 个差异蛋白质在术后 7 ~ 10 d 其含量均呈上升趋势，大部分在术后 30 ~ 40 d 恢复至对照组水平。第 二组为 12 个差异蛋白质，它们在 A1 组与 A3 组比较中有显著差异，而且在 A1、A2 及 A3 组中含量不断上 升，在术后 30 ~ 40 d 时恢复至对照组水平。结论 第一组差异蛋白质在宫颈鳞癌和对照组之间差异有统 计学意义，可作为区分宫颈鳞癌与正常人生物学指标，同时两组差异蛋白质技术在宫颈鳞癌患者手术前 后三个不同时期均有明显差异，预测它们能作为宫颈鳞癌手术疗效评价和术后病情监测的指标。

Objective To study serum differential proteins in patients with cervical squamous cell carcino⁃ ma at three different periods of pre⁃ and post⁃operation and to compare these proteins with those in a control group. Methods The SELDI⁃TOF⁃MS and weak cation binding microarray（CM10）were used to dynamically analyze serum protein fingerprints in patients with cervical squamous cell carcinoma before operation（group A1），on postsurgical days 7 ~ 10（group A2）and 30 ~ 40（group A3），and in a control group. The changes in protein fingerprints at different periods were compared and differential proteins were screened. Results The first group contained 16 differential proteins between cervical squamous cell carcinoma and the control group，12 of differed significantly between groups A1 and A2. The contents of these 16 differential proteins showed an upward trend on postoperative days 7 ~ 10，and most of them returned to the level of the controls on days 30 ~ 40. The second group contained 12 differential proteins，which were significantly different between groups A1 and A3，and their levels were increasing in groups A1，A2 and A3，and then returned to the control level on days 30 ~ 40. Conclusions The differential proteins in the first group had significantly statistical differences between cervical squamous cell carcinomas and the controls，which could be used as a biological indicator. At the same time，the proteins in both groups were significantly distinguished during three different periods of pre⁃ and post⁃operation in patients with cervical squamous carcinoma. Detection of differential proteins could be applied as indicators for assessing the efficacy of surgical treatment for cervical squamous carcinoma and for monitoring postoperative condition.