目的 沙眼衣原体主要外膜蛋白（major outer membrane protein，MOMP）在维持沙眼衣原体结构稳定性和衣原体感染宿主细胞过程中发挥重要作用，为深入研究 MOMP 蛋白的功能及作用机制，使用人工合成多肽制备 MOMP 多克隆抗体。方法 分析沙眼衣原体 MOMP 的抗原表位，根据 15 个沙眼衣原 体血清型 MOMP 保守肽段，设计并合成 MOMP 特异性肽段，与血蓝蛋白偶联后免疫新西兰兔。采用免疫 亲和层析纯化免疫血清，应用 SDS⁃PAGE 和 ELISA 评估血清抗体纯度及效价，利用 Western blot 和免疫荧光 染色鉴定 MOMP 抗体特异性。结果 纯化后的血清 IgG 抗体纯度为 89.6%，MOMP 抗体效价达 1∶12 800， Western blot 结果显示抗体与 8 个血清型沙眼衣原体蛋白在 40 kDa 处特异性结合，免疫荧光染色证实该抗 体可特异性结合沙眼衣原体。此外，MOMP 抗体与进口商品化抗体明显共定位于宿主细胞内，且两种抗体荧光强度相当。结论 本研究成功制备效价高、特异性好的沙眼衣原体 MOMP 的兔多克隆抗体，为后 续深入研究MOMP 的生物学功能提供了有力工具。

Objective The major outer membrane protein（MOMP）of Chlamydia trachomatis（Ct）is essential for Ct structural stability and infection of the host cell. MOMP polyclonal antibody was created using the synthetic peptide to further investigate the function and mechanism of MOMP protein. Methods Ct MOMP antigenic epitope was investigated. MOMP⁃specific peptide was designed and synthesized based on the conserved peptides of 15 different Ct serotype MOMPs，and New Zealand white rabbits were immunized after coupling with hemocyanin. MOMP⁃specific peptide was designed and synthesized based on the conserved peptides of 15 different Ct serotype MOMPs，and New Zealand white rabbits were immunized after coupling with hemocyanin.Immunoaffinity chroma⁃ tography was used to separate the immune serum. SDS⁃PAGE was used to determine the purity of the IgG antibody， and ELISA was used to determine the titer of the MOMP antibody. Furthermore，Western blot and immunofluores⁃ cence staining were used to determine the specificity of the MOMP antibody. Results The purity of IgG antibody was 89.6%，and the titer of MOMP antibody was 1∶12800. The results of Western blot showed that the antibody specifically bound to 8 serotypes of Ct protein at 40kDa，and indirect immunofluorescence staining showed that the antibody could specifically combined with Ct MOMP. In addition，the immunofluorescence results showed that the MOMP antibody and the imported commercial antibody were obviously co ⁃localized in the host cells ，and the fluorescence intensities of the two antibodies were comparable. Conclusions This study successfully prepared arabbit polyclonal antibody with high titer and good specificity against Ct MOMP ，providing a useful tool for future research on the biological function of MOMP.