关键词: PINK1, 胃癌, 细胞凋亡, 化疗敏感性, 奥沙利铂, 耐药

Abstract:

Objective The aim of this research was to investigate the effects of PTEN⁃induced kinas 1（PINK1）on the malignant biological behavior of gastric cancer（GC）. Methods PINK1 expression was downreg⁃ulated by small interfering RNA（siRNA）in GC cells. MTT assay，Wound healing assay and Transwell assay wereconducted to explore the role of PINK1 in GC cells′ proliferation and migration. Survival analysis and MTT experimentswere applied subsequently to find out the effect of PINK1 on chemosensitivity of oxaliplatin. Results Down⁃regulation of PINK1 significantly inhibited the proliferation，migration and invasion of gastric cancer cells. Inhibitionof PINK1 in GC cells could promote the production of reactive oxygen species（ROS）and induced apoptosis⁃related geen Bcl⁃2 upregulationand furtherly induce GC cells deaths. Finally，PINK1 downregulation effectivelyincreased the drug sensitivity of GC cells to oxaliplatin，which highly suggested a chemoresistance role of PINK1 inGC. Conclusion PINK1 may be a potential therapeutic target for GC；down⁃regulation of PINK1 could inhibit themalignant biological behavior of GC cells，enhance the sensitivity of oxaliplatin，and synergistic sensitization withchemotherapy.

Key words: PINK1, gastric cancer, apoptosis, chemosensitivity, oxaliplation, chemo?resistance