关键词:

骨巨细胞瘤, 基质细胞, MALAT1, miR?30, 生长, 迁移和侵袭

Abstract:

Objective To elucidate whether MALAT1 regulates the growth，migration，and invasion of giant cell tumor stromal cells through miR⁃30. Methods Collect the samples of cancer and para⁃cancer in clinical patients with giant cell tumor of bone for fluorescence quantitative PCR，to detect the expression level of MALAT1 and miR⁃30；culture primary cells of giant cell tumor of bone to obtain purified primary stromal cell line；construct MALAT1 overexpression plasmid，synthesize miR ⁃30 mimic and inhibitor；overexpress MALAT1 plasmid and/or miR⁃30 related fragments in cells，observe cell growth by MTT experiment，observe migration and invasion by tran⁃ swell experiment. Results Compared with the adjacent tissues，the expression level of MALAT1 was significantly decreased in giant cell tumor of bone tissue，while the level of miR ⁃ 30 was increased；bioinformatics analysis found that MALAT1 showed matched sequence interacting with miR⁃30，and the luciferase reporter gene reporter showed that overexpression of miR ⁃30 can significantly inhibit the luciferase signal of MALAT1 WT，but not for MUT mutant plasmids；overexpression of MALAT1 or miR⁃30 inhibitor alone inhibited the growth，migration，and invasion of stromal cells；and downstream antagonism experiments showed that overexpression of MALAT1 counter⁃ acted the promotion effect induced by miR⁃30 mimic. Conclusion MALAT1 and miR⁃30 are involved in the devel⁃ opment and regulation of bone giant cell tumor stromal cells；MALAT1 inhibits the proliferation，migration，and invasion of bone giant cell tumor cells by regulating miR⁃30.

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