实用医学杂志 ›› 2021, Vol. 37 ›› Issue (1): 41-45.doi: 10.3969/j.issn.1006⁃5725.2021.01.009

• 基础研究 • 上一篇    下一篇

辛伐他汀联合机械牵拉激活Wnt/β⁃catenin 信号通路促进大鼠骨髓间充质干细胞成骨分化

陈斌伟, 刘圣曜, 黄帅, 王斌 ,陈元鸿   

  1. 广州医科大学附属第二医院(广州 510260)
  • 出版日期:2021-01-10 发布日期:2021-01-10
  • 通讯作者: 刘圣曜 E⁃mail:liushengyao12@163.com
  • 基金资助:
    广东省自然科学基金资助项目(编号:2020A1515010211);广州市卫生健康科技项目(编号:20191A011081)

Simvastatin promotes the osteogenic differentiation of BMSCs induced by mechanical stretch via Wnt/β ⁃ catenin signaling pathway

CHEN Binwei,LIU Shengyao,HUANG Shuai,WANG Bin,CHEN Yuanhong
  

  1. The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China
  • Online:2021-01-10 Published:2021-01-10
  • Contact: LIU Shengyao E⁃mail:liushengyao12@163.com

摘要:

目的 探讨辛伐他汀联合机械牵拉对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的影响及 Wnt/β⁃catenin 通路在其中的作用。方法 实验分为对照组、辛伐他汀组、 机械牵拉组及辛伐他汀+机械牵拉组,利用碱性磷酸酶(ALP)活性及 RT⁃PCR 检测各组 BMSCs ALP 活性 ALP、OCN、Col 1a、RUNX2 基因表达,利用 RT⁃PCR Western blot 检测β⁃catenin 通路相关基因和蛋白的 表达,并探讨 DKK1 处理后辛伐他汀联合机械牵拉对成骨分化的影响。结果 与对照组比,辛伐他汀、机械牵拉及辛伐他汀+机械牵拉均能增加 ALP 活性及成骨基因表达,并能增加β⁃catenin 基因和蛋白表达,且 以辛伐他汀+机械牵拉组升高最明显;使用 Wnt/β⁃catenin 信号通路抑制剂 DKK1 能减弱辛伐他汀+机械牵 拉组促成骨分化效果。结论 辛伐他汀及机械牵拉均促进 BMSCs 成骨分化,且辛伐他汀协同机械牵拉通 过激活Wnt/β⁃catenin 通路促进BMSCs 

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Abstract:

Objective To investigate the effect of simvastatin combined with mechanical stretch on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)and explore its mechanisms. Methods simvastatin group,mechanical stretch group and simvastatin+mechanical stretch group in the study. ALP activity and RT ⁃ PCR were used to investigate the effect of simvastatin,mechanical stretch and simvastatin + mechanical stretch on osteogenic differentiation of BMSCs and RT⁃PCR and WB were used to investigate the expression of β ⁃catenin in each group. Results The level of osteogenic relative genes was up⁃regulated and the expression of β ⁃catenin protein was increased in each group when compared with those in the control group,especially in simvastatin+mechanical stretch group. DKK1 partly eliminated osteogenic differentiation of BMSCs in simvastatin+ mechanical stretch group. Conclusion Both simvastatin and mechanical stretch promote the osteogenic differentia⁃ tion of BMSCs. Simvastatin promotes the osteogenic differentiation of BMSCs induced by mechanical stretch via 

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