Objective To investigate the influencing factors of available blastocysts formation in chromo⁃ some balanced translocation carriers. Methods The clinical data of 421 chromosome balanced translocation cycles of 331 couples undergoing preimplantation genetic testing（PGT）in the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University from January 2017 to December 2021 were retrospectively analyzed in this study. According to the ISCN standard of human chromosome grouping，the enrolled subjects were divided into group A and non⁃Group A. According to the location of the breakpoints，the enrolled subjects were sub⁃divided into group qq，group pp and group pq. Multiple linear regression analysis was used to analyze the independent factors affect⁃ ing available blastocyst formation rate. Results After excluding all confounding factors，it was found that：（1）In reciprocal translocation subjects，the available blastocysts formation rate in group A was significantly lower than that in non⁃group A（B = -0.089，P = 0.001），while sex had no significant effect on the rate of available blastocyst formation（P = 0.713）.（2）In the subjects with Robertsonian translocation，there was no significant difference in the rate of available blastocyst formation between the DD and DG groups（P = 0.261）and sex was not an indepen⁃ dent factor affecting the rate of available blastocyst formation（P = 0.510）.（3）In the balanced translocation popu⁃ lation，translocation type was not an independent factor affecting the formation rate of available blastocysts（P = 0.715）. Conclusion The type of translocation does not affect the formation of available blastocysts in the carriers of balanced translocation. In reciprocal translocation patients，the chromosome length affects the rate of available blastocyst formation. Sex and chromosome type of Robertsonian translocation carriers have no significant effect on the formation rate of available blastocysts.