关键词:

微 RNA?216a, Janus 激酶 2, 信号转导和转录激活因子 3, 卵巢癌细胞, 转移, 上皮-间质转化

Abstract:

Objective To explore whether miRNA⁃216a（mir⁃216a）inhibits the metastasis and epithelial⁃ mesenchymal transition（EMT）of ovarian cancer（OC）cells by regulating the Janus kinase 2（JAK2）/signal trans⁃ ducer and activator of transcription 3（STAT3）pathway. Methods Detection of miR ⁃ 216a expression levels in OC tissues and cells by qRT⁃PCR. Transwell were used to detect the ability of migration and invasion. Western blot and immunofluorescence were used to detect the expression levels of EMT⁃related proteins. The expression levels of JAK2/STAT3 pathway related proteins were analyzed by Western blot. Results The expression of miR⁃216a was down⁃regulated in OC tissues and cells（P < 0.05）. Up⁃regulated miR⁃216a impaired OC cell migration，invasion and EMT and simultaneously inhibited JAK2/STAT3 pathway activation（P < 0.05）. Overexpressed JAK2 reversed the effects of up⁃regulated miR⁃216a on the above indicators（P < 0.05）. Conclusion miR⁃216a may inhibit the metastasis and EMT of OC cells by suppressing JAK2/STAT3 pathway activation. 

Key words:

"> microRNA ? 216a, Janus kinase 2, signal transducer and activator of transcription 3, ovarian cancer cells, metastasis, epithelial?mesenchymal transition